|
|||||
|
|
| 利用卡那霉素对花粉管通道法转基因大豆的筛选研究 | |
| 引用本文： | 徐鹏飞,张淑珍,吴俊江,韩英鹏,张大勇,李文滨,杨传平. 利用卡那霉素对花粉管通道法转基因大豆的筛选研究[J]. 大豆科学, 2006, 25(3): 275-278 |
| 作者姓名： | 徐鹏飞 张淑珍 吴俊江 韩英鹏 张大勇 李文滨 杨传平 |
| 作者单位： | 1. 东北农业大学大豆研究所教育部大豆生物学重点实验室,哈尔滨,150030 2. 黑龙江省农业科学院,哈尔滨,150086 3. 东北林业大学,哈尔滨,150040 |
| 基金项目： | 黑龙江省博士后科研启动基金，黑龙江省科技厅科技计划 |
| 摘 要： | 用卡那霉素对花粉管通道法获得的东农46转化当代种子进行初步筛选,结果表明:400ppm卡那霉素能有效抑制非转化大豆植株的正常生长;经Gus染色和PCR检测,从中可以筛选到阳性植株.可见,用此法对转化植株进行初步筛选是经济有效的. |
| 关 键 词： | 大豆 花粉管通道法 卡那霉素 |
| 文章编号： | 1000-9841（2006）03-0275-04 |
| 收稿时间： | 2005-10-12 |
| 修稿时间： | 2005-10-12 |
THE STUDY ON THE SCREENING OF TRANSGENE SOYBEAN BY WAY OF POLLEN-TUBE USING KANAMYCIN |
|
| Xu Pengfei,Zhang Shuzhen,Wu Junjiang,Han Yingpeng,Zhang Dayong,Li Wenbin,Yang ChuanPing. THE STUDY ON THE SCREENING OF TRANSGENE SOYBEAN BY WAY OF POLLEN-TUBE USING KANAMYCIN[J]. Soybean Science, 2006, 25(3): 275-278 | |
| Authors: | Xu Pengfei Zhang Shuzhen Wu Junjiang Han Yingpeng Zhang Dayong Li Wenbin Yang ChuanPing |
| Affiliation: | 1. Soybean Research Institute of Northeast Agricultural University, Harbin 150030; 2. Heilongjiang Acadeng of Agricultural Sciences, Harbin 150086; 3. North-east Forestry University, Harbin 150040 |
| Abstract: | Kanamycin was used to screen To seeds of transgene Dongnong 46 by way of pollen-tube. The resuits showed that 400ppm kanamycin could inhibit growth of non-transgene plants, and positive plants could be gotten using Gus and PCR detection. It was proved that this method wa economic and effective in screening the transgene plants. |
| Keywords: | Soybean Pollen-tube way Kanamycin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |