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| 虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆 | |
| 引用本文： | 王辉,张秀清,胡玉红,孙君社. 虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆[J]. 华北农学报, 2010, 25(1) |
| 作者姓名： | 王辉 张秀清 胡玉红 孙君社 |
| 作者单位： | 1. 中国农业大学,食品科学与营养工程学院,北京,100083;河北师范大学,生命科学学院,河北,石家庄,050016 2. 中国农业大学,食品科学与营养工程学院,北京,100083 3. 河北师范大学,实验中心,河北,石家庄,050016 |
| 摘 要： | 运用SMART技术成功构建了虎纹蛙(Hoplobatrachus rugulosus)皮肤组织全长cDNA文库。根据蛙科动物抗菌肽的信号肽保守序列设计简并引物,以PCR法成功从文库中随机挑选的阳性克隆中筛选出2个抗菌肽cDNA序列,命名为tigerinin-HR1和tigerinin-HR2。这2个抗菌肽cDNA序列是首次从虎纹蛙皮肤组织中克隆出的抗菌肽编码基因。 |
| 关 键 词： | 虎纹蛙 cDNA文库 抗菌肽 |
Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs |
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| WANG Hui,ZHANG Xiu-qing,HU Yu-hong,SUN Jun-she. Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(1) | |
| Authors: | WANG Hui ZHANG Xiu-qing HU Yu-hong SUN Jun-she |
| Abstract: | A full length cDNA library of Hoplobatrachus rugulosus skin tissue was constructed with the SMART technique.A special primer was designed from a highly conserved domain of the signal peptide of antimicrobial peptide encoding cDNAs from ranid frogs.Two different cDNA sequences encoding precursor proteins named tigerinin-HR1 and tigerinin-HR2 were screened from the library by PCR.tigerinin-HR1 and tigerinin-HR2 were the first found encoding cDNA sequences of antimicrobial peptide from H.rugulosus. |
| Keywords: | tigerinin Hoplobatrachus rugulosus cDNA library Antimicrobial peptides tigerinin |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |