| 三角梅总DNA提取方法比较研究 |
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| 引用本文： | 黄彦晶,吴少华,李房英,曾黎辉. 三角梅总DNA提取方法比较研究[J]. 现代农业科技, 2012, 0(24): 166-168 |
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| 作者姓名： | 黄彦晶 吴少华 李房英 曾黎辉 |
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| 作者单位： | 1. 福建农业职业技术学院园艺园林系,福建福州,350119
2. 福建农林大学园艺学院 |
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| 摘 要： | 为了选择一种快速、有效、简单的三角梅总DNA的提取方法,分别采用DNA提取试剂盒法、CTABmini法、改良SDS-KAC法、CTAB大量法提取三角梅嫩叶片的总DNA,用紫外分光光度计、琼脂糖凝胶电泳对所提取的总DNA进行检测。结果表明：CTABmini法提取的三角梅总DNA纯度高,浓度高,并且简单省时。CTABmini法是较为理想的三角梅总DNA提取方法,该法提取的总DNA适用于三角梅的分子生物学研究。
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| 关 键 词： | 三角梅 总DNA 提取方法 |
Comparative Study on Total DNA Extraction Methods of Bougainvillea spectabilis Willd. |
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| HUANG Yan-jing,WU Shao-hua, LI Fang-ying,ZENG Li-hui. Comparative Study on Total DNA Extraction Methods of Bougainvillea spectabilis Willd.[J]. Modern Agricultural Sciences and Technology, 2012, 0(24): 166-168 |
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| Authors: | HUANG Yan-jing WU Shao-hua LI Fang-ying ZENG Li-hui |
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| Affiliation: | 1 Department of Horticulture and Garden,Fujian Vocational College of Agriculture,Fuzhou Fujian 350119;2 Department of Horticulture,Fujian Agriculture and Forestry University) |
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| Abstract: | To find a rapid,simple and effective total DNA extraction method for Bougainvillea spectabilis willd.,four methods including DNA Isolation Kit method,CTAB mini method,improved SDS-KAC method,improved CTAB method were compared.The total DNA was detected by spectrophotometer,agarose gel electrophoresis.The results showed that CTAB mini method was simple,timesaving,high-yield,high-purity.CTAB mini method was optimal for total DNA extraction in Bougainvillea spectabilis Willd.,which was suitable for molecular biology researches. |
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| Keywords: | Bougainvillea spectabilis Willd. total DNA extraction method |
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