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| 利用流式细胞术检测H4亚型禽流感病毒感染细胞 | |
| 引用本文： | 高明燕,胡茂志,吴艳涛,王志亮,焦新安,刘秀梵.利用流式细胞术检测H4亚型禽流感病毒感染细胞[J].扬州大学学报(农业与生命科学版),2007,28(4):10-12. |
| 作者姓名： | 高明燕 胡茂志 吴艳涛 王志亮 焦新安 刘秀梵 |
| 作者单位： | 1. 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 2. 扬州大学,江苏省物质微区与性能测试服务中心,江苏,扬州,225009 3. 农业部动物检疫所,外来动物疾病诊断中心,山东,青岛,266032 |
| 基金项目： | 江苏省高校自然科学重大基础研究项目(05KJA23016) |
| 摘 要： | 以不同稀释度的H4亚型禽流感病毒(AIV)感染MDCK细胞,分别在添加与不添加TPCK-胰酶的培养基中培养36 h后收集细胞。经固定后,加入抗H4亚型AIV的单抗2C11,继续加入荧光标记的抗鼠Ig抗体,用流式细胞仪(FCM)分析不同条件下病毒感染的细胞,并用血凝(HA)试验检测细胞培养上清中病毒的HA效价。FCM结果表明:不论加TPCK-胰酶与否,均能引起病毒的感染,且随着感染剂量的增加,含有病毒细胞的比率增加。相比而言,加胰酶后,低剂量感染便可引起病毒在MDCK细胞内大量增殖,并能检测到细胞上清中的病毒。FCM与HA试验相比,FCM试验更敏感,更能反应病毒在感染早期的增殖情况。 |
| 关 键 词： | 禽流感病毒 血凝试验 流式细胞术 |
| 文章编号： | 1671-4652(2007)04-0010-03 |
| 修稿时间： | 2007年9月10日 |
Detection of aian influenza virus-infected cells by flow cytometry |
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| GAO Ming-yan,HU Mao-zhi,WU Yan-tao,WANG Zhi-liang,JIAO Xin-an,LIU Xiu-fan.Detection of aian influenza virus-infected cells by flow cytometry[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2007,28(4):10-12. | |
| Authors: | GAO Ming-yan HU Mao-zhi WU Yan-tao WANG Zhi-liang JIAO Xin-an LIU Xiu-fan |
| Abstract: | MDCK cells,which were infected by H4 subtype avian influenza virus with different diluted concentration were cultured in the culture medium containing TPCK-trypsin or not,seperately.36 hours later,cells were fixed and analysed by Flow Cytometry(FCM) after stained with monoclonal antibody 2C11.Simultaneously,the HA test was subjected to detect virus in cell culture.FCM result indicated that the percent of virus-infected cells were detected and consistent to the infectous dose,whether containing TPCKtrypsin or not.Comparably,after low dose infection with TPCK-trypsin,large amounts of virus-infected cells were detected and HA value of virus in cell culture increased evidently.FCM method was more sensitive than HA test and could be used to detect the early infection of virus. |
| Keywords: | avian influenza virus hemagglutinin test flow cytometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |