Kinetics of acid phosphatase in calcium chloride extractable soil organic matter |
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Authors: | S. Marinari G. Masciandaro B. Ceccanti S. Grego |
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Affiliation: | aDipartimento di Agrobiologia e Agrochimica, Università della Tuscia, via S. Camillo de Lellis, 01100, Viterbo, Italy;bIstituto per lo Studio degli Ecosistemi – Sezione di Chimica del Suolo, CNR, 56125 Pisa, Italy |
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Abstract: | The aim of this work was to compare the kinetic parameters of acid phosphatase (EC 3.1.3.2.) extracted from two forest soils under oak or pine. Soil was extracted with 4 mM CaCl2 and the extract was divided into two fractions by filtration: one >0.2 μm containing microbial cells and soil particulates, and the other <0.2 μm containing fine particles and dissolved organic compounds of soil. The >0.2 μm fraction had higher Km (0.26–0.82 vs. 0.12–0.39) and Vmax (0.07–0.79 vs. 0.06–0.16) values than the <0.2 μm fraction, indicating a higher enzyme-substrate affinity and smaller amount of enzyme in fine particles and dissolved organic matter. |
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Keywords: | Acid phosphatase Calcium chloride extractable organic matter Enzymes kinetics Km Vmax |
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