| 低能离子束介导转基因小麦叶片蛋白指纹分析 |
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| 引用本文： | 姬生栋,秦广雍,等.低能离子束介导转基因小麦叶片蛋白指纹分析[J].麦类作物学报,2001,21(4):52-55. |
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| 作者姓名： | 姬生栋 秦广雍 |
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| 作者单位： | 1. 河南师范大学生命科学学院,
2. 郑州大学物理工程学院, |
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| 基金项目： | 国家九五重点科技攻关项目 ( 96 -5 38-0 1-0 7)和河南省创新人才基金资助项目 ( 5 2 0 448)。 |
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| 摘 要： | 用SDS－PAGE电泳方法研究了低能离子束介导转大豆DNA小麦灌浆期叶片中蛋白图谱变化，结果表明，离子束介转基因小麦部分个体的叶片蛋白图谱与对照有显著差异，各处理的蛋白图谱中，带型变化主要集中在157，104，83，36，35，34，28和23kD等条带；相对稳定的蛋白质条带分别为47，44，41，40，39，31和24kD。各处理间蛋白图谱存在一定的差异。导入DNA时间对蛋白质图谱有显著影响。
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| 关 键 词： | SDS－PAGE 小麦 离子束 DNA 蛋白质 转基因 指纹分析 |
| 文章编号： | 1009-1041(2001)04-0052-04 |
| 修稿时间： | 2001年1月31日 |
Analysis of Fingersprint of Transgenic Wheat Leaves Irradiated with Low Energy |
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| JI Sheng dong,LI Ji xue,ZHAO Jun jie,XU Cun shuan,QIN Guang yong,WANG Wei dong,HUO Yu ping.Analysis of Fingersprint of Transgenic Wheat Leaves Irradiated with Low Energy[J].Journal of Triticeae Crops,2001,21(4):52-55. |
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| Authors: | JI Sheng dong LI Ji xue ZHAO Jun jie XU Cun shuan QIN Guang yong WANG Wei dong HUO Yu ping |
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| Abstract: | The protein graph of wheat leaves irradiated soybean DNA during the milk stage was studied with SDS PAGE. The results showed that the protein graph of wheat leaves irradiated exogenous gene was much different from control wheat leaves, and there were many differences between different treatments. Of these proteins, the 157, 104, 83, 36, 35, 34, 28 and 23 kD proteins changed most greatly after different treatments, and under the same conditions, the 47, 44, 41, 40, 39, 31 and 24 kD proteins were stable relatively. And the transqenic time affected the protein graph. |
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| Keywords: | SDS PAGE Wheat Irradiation DNA Protein |
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