整体显示小鼠骨骼标本的制作试验

本试验分别采用4种不同处死方法和3种染色方法，制作小鼠骨骼透明标本。结果表明，水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油-氨水漂白、甘油逐级透明的制作方法，获得的透明标本效果最佳。控制温度，可以使标本的固定速度加快，缩短制作时间。

Study on facture of transparent specimen in mice

The results of making transparence specimen of mouse skeleton were compared respectively, making mouse death in 4 different methods and dyeing them in 3 methods. The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen. Temperature controlling could reduce the time of specimen-fixed.