| STR分型技术在马亲子鉴定中的应用 |
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| 引用本文： | 黄娅琳.STR分型技术在马亲子鉴定中的应用[J].河南农业科学,2010(7). |
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| 作者姓名： | 黄娅琳 |
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| 作者单位： | 南京森林公安高等专科学校,江苏,南京,210046;国家林业局,野生动植物物证鉴定中心,江苏,南京,210046 |
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| 基金项目： | 南京森林公安高等专科学校教研基金资助课题 |
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| 摘 要： | 利用STR分型技术进行马亲子鉴定。取VHL20、HTG4、AHT4、HMS7、HTG6、HMS6、AHT5、ASB2、HTG7、HMS3、HMS2共11个STR(短串联重复序列)基因座,运用PCR技术对争议小马、嫌疑母马以及无关对照马的DNA进行扩增,扩增产物经遗传分析仪检测,用GeneMap-per片段分析软件对检测结果进行分析。结果表明,争议小马与嫌疑母马无亲子关系,STR基因分型技术能够准确、快速地进行马亲子鉴定,所用的STR位点具有较高的鉴别效率。
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| 关 键 词： | STR分型 马 亲子鉴定 |
Application of STR Loci Analysis in Parentage Identification of Horse |
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| HUANG Ya-lin.Application of STR Loci Analysis in Parentage Identification of Horse[J].Journal of Henan Agricultural Sciences,2010(7). |
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| Authors: | HUANG Ya-lin |
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| Abstract: | To apply short tandem repeat(STR) loci in the parentage identification of horse,eleven short tandem repeat(STR) loci including VHL20,HTG4,AHT4,HMS7,HTG6,HMS6,AHT5,ASB2,HTG7,HMS3,and HMS2 on DNA of three horses were amplified by PCR.The PCR products were further separated and analyzed in a genetic analyzer(AB 3130xl).The results showed that the paternity probability between the young horse and the mare was ruled out.This indicates that most of the STR locus used presently is high performance and STR loci analysis was a validation method for parentage identification in horse. |
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| Keywords: | Short tandem repeat(STR) loci Horse Parentage identification |
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