茄类内生菌的分离及拮抗细菌的筛选

1材料与方法1．1植株及病原菌 健康的番茄、辣椒及茄子植株，采自茂名市郊区菜园；茄科雷尔氏菌（Ralstonia solanacearum）由茂名学院生物技术与食品工程实验中心分离得到。

Isolation of Endophyte and Screening for Antagonistic Bacteria in Solanaceae

[Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [Method] According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified endophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.