| 利用RAPD分子标记对30份山茶属种质资源进行分类分析 |
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| 引用本文： | 晏嫦妤,黄华林,李家贤,何玉媚.利用RAPD分子标记对30份山茶属种质资源进行分类分析[J].安徽农业科学,2014(16):4962-4963,4968. |
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| 作者姓名： | 晏嫦妤 黄华林 李家贤 何玉媚 |
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| 作者单位： | 广东省农业科学院饮用植物研究所,广东广州510640;广东省茶树种质资源创新利用重点实验室,广东广州510640;广东省农业科学院饮用植物研究所,广东广州,510640 |
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| 基金项目： | 广东省科技计划项目(2011B060400026);广东省农业科学院院长基金(201439) |
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| 摘 要： | 目的]对30份山茶属种质资源进行分类分析.方法]利用RAPD分子标记技术研究30份山茶属种质资源的遗传多样性,并进行分类分析.结果]17个RAPD引物共扩增出138条带,多态性条带为129条,多态性比率为93.5％,材料间的遗传相似系数（GS）变化范围为0.605 ～ 0.855.结论]利用NTSYS软件对RAPD扩增结果进行聚类分析,可将30份资源分为5大类群,其分类结果与张宏达分类体系基本一致.
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| 关 键 词： | 山茶属种质资源 RAPD 分类 |
Classification and Analysis of 30 Camellia Germplasm Resources by Using RAPD Molecular Markers |
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| Institution: | YAN Chang-yu( 1.Drinkable Plants Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640; Guangdong Key Laboratory of Tea Plant Resources Innovation ＆ Utilization, Guangzhou, Guangdong 510640;) |
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| Abstract: | Objective] To classify and analyze 30 Camellia germplasm resources.Method] Using RAPD molecular markers,the genetic diversity of 30 Camellia germplasm resources was studied and classified.Result] The result showed that 138 bands were amplified by 17 RAPD primers and 129 polymorphic bands （93.5％）.The variance range of genetic similarity （GS） among 30 accessions of Camellia germplasm was 0.605-0.855.Conclusion] The dendrogram was constructed by using the NTSYS software based on RAPD data indicated that 30 Camellia germplasm were divided into 5 classes,and the classification results were basically the same as the Zhang Hongda classification system. |
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| Keywords: | Camellia germplasm RAPD Classification |
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