影响新疆紫草毛状根生长的因子研究

[目的]研究毛状根生长过程中的影响因子,为新疆紫草毛状根生产紫草素及其衍生物提供技术支持.[方法]采用SH液体培养法,分别考察硝酸钾、酸水解酪蛋白、磷酸二氢铵对毛状根生长的影响.[结果]硝酸钾浓度为3.0 g/L时增殖倍数达6.32倍；在硝酸钾为3.0 g/L、磷酸二氢铵为0.3 g/L时毛状根增殖倍数达10.37倍；当酸水解酪蛋白为0.5 g/L、硝酸钾为3.0 g/L、磷酸二氢铵0.3 g/L三者配合使用时,毛状根增殖倍数平均达12.62倍；培养液pH随着培养时间的延长呈下降趋势,但对毛状根生长没有显著影响；生长末期pH下降幅度为0.41 ～1.85;起始pH高时,末期pH降低的幅度大,起始pH低时末期降低的幅度小.起始pH偏酸有利于毛状根生长；在pH5.8、培养容积为250 mL时,毛状根增殖倍数达15.47倍.[结论]硝酸钾是影响新疆紫草毛状根生长增殖的必要条件,酸水解酪蛋白、硝酸钾、磷酸二氢铵有互作效应.

Research of Effects on Arnebia euchroma(Royle) Johnst Hairy Roots Growth Factors

【Objective】The research intended to investigate the factors that affect hairy roots growth process and provide technical support for production of shikonin and its derivatives on Arnebia euchroma (Royle) Johnst hairy roots.【Method】The effects of hairy roots growth with KNO_3,Casein Hydrolysate and NH_4H_2PO_4 on liquid culture in SH medium were investigated.【Result】Proliferation has increased by 6.32 times under concentratiaon of KNO_3 3.0 g/L;Hairy roots proliferation has increased by 10.37 times under concentration of KNO-3 3.0 g/L and NH_4H_2PO_40.3 g/L;Hairy roots proliferation has increased by an average of 12.62 times under concentration of Casein Hydrolysate 0.5 g/L,KNO_3 3.0 g/L and NH_4H_2PO_4 0.3 g/L used in combination;The medium pH value decreased with the prolongation of culture time,but no significant effects on hairy roots growth were revealed;Last growth stage pH value declined from 0.41 to 1.85;Last growth stage pH value reduced heavily when initial pH value was high,and it reduced lightly when initial pH value was low;Initial pH partial acid was favorable to hairy roots growth;Proliferation has increased by 15.47 times under pH value 5.8 and culture volume 250 mL.【Conclusion】Niter is a necessary condition to influence the growth and proliferation of Arnebia euchroma(Royle) Johnst hairy roots that add KNO_3,and Casein Hydrolysate、KNO_3 and NH_4H_2PO_4 have interaction effect.