芒柄花素对免疫抑制小鼠免疫功能影响的研究

本研究旨在探讨芒柄花素对免疫抑制小鼠免疫功能的影响。将100只昆明种小鼠随机分为5组：空白对照组、免疫抑制组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组，每组20只（雌雄各半），采用灌胃法给药。试验期28 d，试验1~7 d，对照组小鼠灌胃0.6 mL生理盐水，其余各组小鼠均灌胃0.6 mL 40 μg/g体重环磷酰胺（CTX）；试验8~21 d，试验Ⅰ、Ⅱ、Ⅲ组小鼠分别灌胃0.6 mL 50、150、250 μg/g体重芒柄花素溶液，空白对照组与免疫抑制组灌胃0.6 mL生理盐水。试验结束后，测定小鼠脏器指数（胸腺、脾脏）、血清溶血素及IL-2、IL-4含量。采用免疫组化法检测小鼠胸腺、脾脏CD3和CD20阳性淋巴细胞，肝脏CD68阳性KCs细胞。结果表明，环磷酰胺可成功复制小鼠免疫抑制模型。不同剂量芒柄花素均能提高免疫抑制小鼠胸腺和脾脏指数、血清溶血素及血清IL-2和IL-4含量，尤其当芒柄花素灌胃剂量为150 μg/g体重时效果最为明显，与免疫抑制组差异极显著（P<0.01）。免疫组化分析结果显示，不同剂量芒柄花素均可提高免疫抑制小鼠胸腺和脾脏CD3阳性T淋巴细胞及CD20阳性B淋巴细胞数量，并促使肝脏CD68阳性KCs细胞增殖，也以试验Ⅱ组效果最显著，与免疫抑制组差异极显著（P<0.01）。综上，芒柄花素可促进小鼠相关淋巴细胞增殖和细胞因子的释放，进而增强机体体液免疫和细胞免疫功能，并可明显促进肝脏固有吞噬细胞KCs增殖。

The Effect of Formononetin on the Immune Function in Immunosuppressed Mice

To investigate the effect of formononetin on the immune function in immunosuppressed mice,100 Kunming mice were randomly divided into five groups (20 in per group,half male and half female):normal control group,immunosuppression group,test groupsⅠ,Ⅱ,and Ⅲ,and the medicines were consecutively given to mice by means of intragastric perfusion.The experimental period was 28 d.The mice in immunosuppression group,test groupsⅠ,Ⅱ,and Ⅲ were all given cyclophosphamide (CTX) with 40 μg/(g·BW) for 1-7 d.From 8 to 21 d,the mice in test groupsⅠ,Ⅱ,and Ⅲ were given the solution of formononetin with 50,150 and 250 μg/(g·BW),respectively.While the other mice were given 0.6 mL physiological saline.After the test,thymus index,spleen index,serum hemolysin and the content of IL-2,IL-4 in serum were detected.The surface receptors CD3,CD20 of lymphocyte and the surface receptors CD68 of KCs were measured by immunohistochemistry.The results showed that the immunosuppressed mice were copied successfully with cyclophosphamid,and the formononetin could increase the serum hemolysin,thymus index,spleen index,the content of IL-2 and IL-4 in serum in immunosuppressed mice,and the differences between the immunosuppression group and test group Ⅱ were extremely significant (P<0.01).Immunohistochemical analysis showed that the formononetin could increase CD3 positive T lymphocytes and CD20 positive B lymphocytes significantly in thymus and spleen of immunosuppressed mice,and encourage the proliferation of KCs cells in the liver of immunosuppressed mice,and the differences between the immunosuppression group and test group Ⅱ were also extremely significant (P<0.01).The results suggested that formononetin could improve the immune function of immunosuppressed mice by promoting the proliferation of lymphocytes and releasing the cytokines,and promote obviously the proliferation of KCs in liver.