云南热区咖啡种植地红壤细菌群落多样性分析

为了解云南热区咖啡种植地红壤细菌类群的生态分布情况，利用高通量测序技术分别对云南潞江坝（干热区）和芒市（湿热区）5个不同咖啡种植年限的红壤细菌多样性进行分析。16S rDNA测序质控后，获得五个咖啡种植地土样Clean Reads数平均为80 167，细菌物种数目平均为3 118。2个云南咖啡主产区红壤细菌群落划分为39个已知菌门，其中优势门为变形菌门、酸杆菌门、放线菌门、绿弯菌门、芽单胞菌门、拟杆菌门、浮霉菌门、疣微菌门和厚壁菌门。潞江坝与芒市咖啡种植地红壤的细菌群落组成、优势菌属、相对丰度、多样性指数均存在一定差异。其中，潞江坝咖啡种植5和8 a的红壤细菌物种数目和丰富度均高于芒市咖啡种植4和6 a的红壤，但与芒市咖啡种植20 a的红壤无显著差异。随咖啡种植年限的增加，潞江坝或芒市咖啡种植地红壤的细菌优势属均发生了一定变化，但细菌群落的丰富度和多样性无显著差异。该结果为云南热区咖啡种植红壤的细菌类群及其作用研究提供了参考信息，有助于云南咖啡种植的可持续发展。

Diversity of Red Soil Bacterial Community in Coffee-growing Regions of Tropical Yunnan

For better understanding the distribution of red soil bacterial community in tropical coffee-growing regions of Yunnan, diversity of red soil bacterial community with different coffee cultivation years in Lujiangba (dry tropical region of Yunnan) and Mang city (humid tropical region of Yunnan) were investigated by a high-throughput sequencing. The clean reads with an average number of 80 167 and the observed bacterial species with an average number of 3 118 were detected from five soil samples by using 16S rDNA amplicon sequencing technique. A total of 39 bacterial phyla were detected, and Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes, Bacteroidetes, Planctomycetes, Verrucomicrobia and Firmicutes were the most abundant phyla in all red soil bacterial communities. Bacterial community composition, abundance, diversity and dominant genera of red soil in Lujiangba and Mang city were apparently different. The species and richness of red soil bacterial community in coffee-growing region of Lujiangba planted for 5 and 8 a were more and higher than those in Mangshi for 4 and 6 a. But the species and richness of red soil bacterial community in coffee-growing region of Lujiangba had no significant difference with those in Mang city with 20 a cultivation. With increase of coffee cultivation years, the dominant genera of red soil bacterial communities changed, but the bacterial community richness and diversity had no significant difference. These results provided insight into the function of red soil bacterial community in coffee-growing regions of Tropical Yunnan and would contribute to the sustainable development of Yunnan coffee.