甜瓜柱头黄绿颜色相关性状QTL分析

以绿色柱头甜瓜品种MR-1和黄色柱头甜瓜品种TN为亲本,构建F_2群体,利用200个F_2单株为作图群体,基于双亲材料基因组重测序数据开发的SNP-CAPS标记,构建了一张包含12个连锁群、135个CAPS标记的遗传连锁图谱,覆盖基因组长度2 731.97 cM。利用该图谱对甜瓜柱头黄绿颜色相关性状进行QTL分析,共检测到12个与柱头颜色相关的QTL,其中与柱头总叶绿素含量相关QTL 4个,柱头类胡萝卜素含量相关QTL 3个,柱头色卡颜色相关QTL 2个,柱头总叶绿素含量与类胡萝卜素含量比值相关QTL 3个,分布在1、2、4、5、7、8、11、12号连锁群上,可解释4.63%～20.44%的表型变异,其中,调控柱头总叶绿素含量、类胡萝卜素含量和柱头色卡颜色的主效位点分别为SChC-1-1、SCaC-12-1和SCoC-2-1,其贡献率分别为18.78%、12.08%和20.44%。

QTL Analysis of Melon Stigma Color

In this experiment，200 F₂ populations were obtained from green stigma melon ‘MR-1’and yellow stigma melon ‘TN’ as parents，and based on resequencing the SNP-CAPS markers and BSA technology were developed for construction of the molecular map．A genetic linkage map containing 12 linkage groups and 135 CAPS markers was constructed，covering a genome length of 2 731.97 cM．QTL analysis was conducted on the related characters of melon stigma color（yellow or green） by this map．Twelve QTLs related to stigma color were obtained．There were 4 QTLs related to stigma total chlorophyll content，3 QTLs related to stigma carotenoid content，2 QTLs related to stigma color card，and 3 QTLs related to the ratio of stigma total chlorophyll content to stigma carotenoid content，distributing in 1，2，4，5，7，8，11，12 on the linkage group，explaining 4.63%-20.44% phenotypic variation．The main effect sites regulating stigma total chlorophyll content，stigma carotenoid content and stigma color card were SChC-1-1，SCaC-12-1and SCoC-2-1．Their contribution rates were 18.78%，12.08%，20.44%，respectively．