碱性成纤维细胞生长因子在苜蓿中的表达

为了降低bFGF（basic fibroblast growth factor）的生产成本，结合植物生物反应器的优点，就bFGF在转基因苜蓿中的表达进行了探索。将bFGF插入植物表达载体pBⅡ21中，获得了含有bFGF基因的植物表达pBIcbFGF，再将pBIcbFGF利用冻融法转到农杆菌中。利用农杆菌介导法将基因转化保定苜蓿，转基因苜蓿在TM-1培养基＋20mg／L卡那霉素（Kan）＋200mg／L特美汀（Tim）中诱导分化，在生根培养基中生根，获得再生植株。再生植株通过PCR检测、RT—PCR及Western blot证实外源基因已经在苜蓿中成功表达。获得含有目的蛋白的阳性植株。为苜蓿作为植物生物反应器生产bFGF奠定了理论及技术基础。

Expression of Basic Fibroblast Growth Factor in Alfalfa

In order to reduce the production costs of bFGF, we explored the expression of bFGF in transgenic alfalfa.A plant expression vector pBIcbFGF was constructed by inserting bFGF gene into plasmid pBII21, and transformed into Agrobacterium using Freeze-thaw method.Transformed into Baoding alfalfa using Agrobacterum-mediated transformation to obtain transgenic alfalfa.Genetically modified alfalfa at TM-1 medium +20 mg/L Kan+ 200 mg/L Tim in the induction of differentiation, in the rooting medium induced root, to...