| HPLC测定利咽解毒颗粒中黄芩苷的含量 |
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| 引用本文： | 陈强,周浓,张海珠. HPLC测定利咽解毒颗粒中黄芩苷的含量[J]. 安徽农业科学, 2010, 38(35): 20029-20030. DOI: 10.3969/j.issn.0517-6611.2010.35.058 |
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| 作者姓名： | 陈强 周浓 张海珠 |
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| 作者单位： | 成都军区机关医院,四川成都,610011;大理学院药学院,云南大理,671000 |
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| 摘 要： | [目的]建立HPLC法测定利咽解毒颗粒中黄芩苷含量的方法。[方法]色谱柱为Agilent zorbax SB-C18柱（4.6 mm×150.0 mm,5μm）,柱温30℃,流动相为甲醇-水-磷酸（V∶V∶V=47∶53∶0.2）,流速为1.0 ml/min,检测波长为280 nm。[结果]在0.5～100.0μg/ml范围内黄芩苷峰面积与浓度呈良好的线性关系（r=0.999 1）,平均回收率为101.14%,RSD值为0.69%（n=9）。[结论]该方法快速、简便、准确,可用于该制剂的质量控制。
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| 关 键 词： | 利咽解毒颗粒 黄芩苷 含量测定 高效液相色谱法 |
Determination of Content of Baicalin in Liyan Jiedu Keli by HPLC |
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| Affiliation: | CHEN Qiang et al(Department of Traditional Chinese Medicine,Military Region Institution Hospital,Chengdu,Sichuan 610011) |
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| Abstract: | [Objective]To establish a HPLC method for the quantitative determination of Baicalin in Liyan Jiedu Keli.[Methods]The HPLC Conditions were as follows:Agilent zorbax SB-C18 column(4.6 mm×150.0 mm,5 μm),mobile phase of methanol-water-phosphoric acid(V∶V∶V=47∶53∶0.2),flow rate of 1.0 ml/min.detection wavelength of 280 nm and column temperature of 30 ℃ [Results]The better linear range of baicalin was 0.5-100.0 μg/ml(r=0.999 1),the mean recovery was 101.14 % with RSD 0.69 %(n=9).[Conclusion]The method is fast,simple and accurate,which can be applied for the quality control of Liyan Jiedu Keli. |
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| Keywords: | Liyan Jiedu Keli HPLC Baicalin Determination |
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