| 秋水仙素处理‘罗田甜柿’获得12倍体再生植株 |
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| 引用本文： | 谷晓峰,罗正荣.秋水仙素处理‘罗田甜柿’获得12倍体再生植株[J].园艺学报,2003,30(3):325-328. |
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| 作者姓名： | 谷晓峰 罗正荣 |
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| 作者单位： | (华中农业大学园艺林学学院，武汉 430070; 湖北民族学院园艺系，恩施 445000) |
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| 基金项目： | 国家自然科学基金项目 (30 0 70 5 2 9)，教育部优秀青年教师资助计划项目，高等学校博士学科专项基金项目 (19990 0 40 0 1) |
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| 摘 要： | 以‘罗田甜柿’试管苗离体叶片为材料，研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响。结果表明：0.3％秋水仙素处理4～6 d，可诱导染色体加倍; 与对照比较, 再生变异植株的气孔孔径增大，密度降低，根尖染色体数2n=12x=180，叶片DNA含量为对照的两倍。LI、LⅡ、LⅢ细胞层均加倍的证据表明，获得的倍性变异株为同质12倍体。
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| 关 键 词： | 柿 秋水仙素 多倍体 再生植株 |
| 文章编号： | 0513-353X(2003)03-0325-03 |
| 收稿时间： | 2002-6-5 |
| 修稿时间： | 2002年6月6日 |
Regeneration of Dodecaploid Plants from in Vitro Leave of'Luotian Tianshi'Persimmon Treated with Colchicine |
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| Gu Xiaofeng,and Luo Zhengrong.Regeneration of Dodecaploid Plants from in Vitro Leave of''''Luotian Tianshi''''Persimmon Treated with Colchicine[J].Acta Horticulturae Sinica,2003,30(3):325-328. |
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| Authors: | Gu Xiaofeng and Luo Zhengrong |
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| Institution: | 1. Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, China;
2. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China |
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| Abstract: | A study of inducing polyploidy by treating'Luotian Tianshi'persimmon in vitro leaflet explants with colchicine solution was performed.The results indicated that mutated plantlets can be induced by treating the leaflet in 0.3% colchicines solution for 4-6 d.Stomata of the mutated plantlets were significantly larger than those of the normal plantlets,but the density of stomata were reduced significantly.The chromosome number of root-tip cell of mutated plantlets was 2n=12x=180,and nuclear DNA content of the mutated plantlets were twice as much as normal hexaploid plantlets.These results suggest that the ploidy of mutated plantlets was autododecaploid. |
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| Keywords: | Persimmon Colchicine Dodecaploid Regeneration |
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