松油烯-4-醇对家蝇几种代谢酶及酚氧化酶的影响

    为进一步探讨松油烯-4-醇的作用机理,以点滴法处理家蝇成虫后,测定兴奋期、痉挛期、麻痹期及复苏期4个中毒阶段试虫的4种代谢酶及酚氧化酶(PPO)活性.结果表明:松油烯-4-醇可明显激活羧酸酯酶(CarE);酸性磷酸酯酶(ACP)表现出先被激活后被抑制,在复苏期又被激活的变化过程,而碱性磷酸酯酶(AKP)被明显抑制,各期酶抑制率分别为8.0%、28.0%、45.0%和33.0%;谷胱甘肽S-转移酶(GSTs)被明显激活,各期酶比活力分别为对照的1.59、1.19、1.26和2.76倍;松油烯-4-醇可显著抑制细胞色素P450O-脱甲基活性,在麻痹期和复苏期的酶抑制率分别为46.91%和60.89%;离体和活体条件下均可显著激活PPO活性.可见,松油烯-4-醇对家蝇代谢酶系的影响与其毒杀作用有关,而中毒试虫的复苏可能与GSTs的激活有关.该化舍物可开发为新型农药或增效剂,在害虫抗性治理中具有潜在的应用价值.

Effects of terpinen-4-ol on four kinds of metabolizing enzymes and polyphenol oxidase in Musca domestica

To study insecticidal mechanism of terpinen-4-ol,the adults of Musca domestica were investigated with terpinen-4-ol by topical application.The activities of carboxyl esterases(CarE),phosphatase,glutathione S-transferase(GSTs),cytochrome P450(P450),and polyphenol oxidase(PPO)of insects tested were determined in all poisoning stages,including exciting stage,convulsing stage,paralysis stage,and recover stage.The result shows that the activity of CarE in houseflies tested was induced by terpinen-4-ol.Acid phosphatase(ACP)was induced in exciting stage,convulsing stage,and recovering stage,but was inhibited in paralysis stage.The activity of alkaline phosphatase(AKP)was inhibited,the inhibition rates were 8.0%,28.0%,45.0%,and 33.0%in exciting stage,convulsing stage,paralysis stage,and recovering stage,respectively.GSTs in houseflies treated were induced,and the specific activities in all four poisoning stages were 1.59,1.19,1.26,and 2.76 times of the control.O-demethylase activity of cytochrome P450 was inhibited by terpinen-4-ol,and the inhibition rates in paralysis stage and recovering stage were 46.91% and 60.89%,respectively.PPO activity was strongly induced by terpinen-4-ol,both in vitro and in vivo.In conclusion,the activities of metabolizing enzymes could have relation with toxicity of terpinen-4-ol against the adults of M.domestica,but the recover of the poisoning insects might be related to GSTs induced.As a new insecticide or synergist,terpinen-4-ol has a potential value in the field of insecticide resistance management.