| 百合系列间杂种多倍体诱导实验初探 |
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| 引用本文： | 吴青青,郑思乡,王继华,许超.百合系列间杂种多倍体诱导实验初探[J].西部林业科学,2010,39(4). |
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| 作者姓名： | 吴青青 郑思乡 王继华 许超 |
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| 作者单位： | 云南农业大学园林园艺学院;贵州省农业科学院园艺研究所;云南省农业科学院花卉研究所; |
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| 基金项目： | 国家科技支撑计划项目，贵州省体改项目，贵州省基金，贵州省省长基金，贵州省农业动植物育种专项，贵州省高层次人才特助项目，贵州省农科院专项 |
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| 摘 要： | 用百合的O/A系列间杂种为实验材料,通过组织培养,比较不同浓度的秋水仙素及不同处理时间长度的诱导效果的差异。结果表明,用浓度为0.05%的秋水仙素处理24 h的效果最佳,突变率高达38.5%。与正常植株相比,变异植株的叶片肥厚,叶面变宽,叶色深绿,气孔显著增大。通过显微镜观察根尖细胞,发现其染色体数目为2n=4x=48。
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| 关 键 词： | 百合 秋水仙素 多倍体 |
Polyploid Induction of Lily Derived from Intersectional Hybrid |
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| WU Qing-qing,ZHENG Si-xiang,WANG Ji-hua,XU Chao.Polyploid Induction of Lily Derived from Intersectional Hybrid[J].Journal of West China Forestry Science,2010,39(4). |
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| Authors: | WU Qing-qing ZHENG Si-xiang WANG Ji-hua XU Chao |
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| Institution: | WU Qing-qing1,ZHENG Si-xiang2,WANG Ji-hua3,XU Chao1(1.College of Horticulture and Landscape,Yunnan Agricultural University,Kunming Yunnan 650201,P.R.China,2.Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550006,3.Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming Yunnan 650205,P.R.China) |
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| Abstract: | Taking lily derived from intersectional hybrids between Oriental hybrids and Asiatic hybrids as experimental materials,the inducing effects of different concentration colchicine and different treating time were compared.The results showed that 0.05 % colchicine and treating time 24 h got the optimum inducing effect,with the inducing percentage of 38.5 %.Compared with the control,the induced plant had thicker and wider leaf,the color of leaf was dark green,and the stoma was increased significantly.Moreover,t... |
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| Keywords: | lily colchicine polyploid |
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