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玉米组织培养体系的研究
引用本文:杜何为,张祖新,郑用琏.玉米组织培养体系的研究[J].湖北农业科学,2003(4):22-24,39.
作者姓名:杜何为  张祖新  郑用琏
作者单位:1. 湖北农学院生命科学学院,湖北,荆州,434025;华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070
2. 华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070
基金项目:湖北省教育厅科研基金项目( 2 0 0 0B0 50 0 5)
摘    要:以玉米的幼胚为外植体,初步研究了培养基、幼胚大小及幼胚的放置方式对愈伤组织诱导的影响。得到了“A188”Ⅱ型愈伤组织,经分化得到再生植株,再生植株的成活率比较高。建立了完善的“A188”幼胚组织培养体系。

关 键 词:玉米  组织培养体系  幼胚  外植体  培养基  放置方式  再生植株  成活率  愈伤组织
文章编号:0439-8114(2003)04-0022-03

A study on technological platform of tissue culture in maize
DU He-wei ,ZHANG Zu-xin ,ZHENG Yong-lian.A study on technological platform of tissue culture in maize[J].Hubei Agricultural Sciences,2003(4):22-24,39.
Authors:DU He-wei    ZHANG Zu-xin    ZHENG Yong-lian
Institution:DU He-wei 1,2,ZHANG Zu-xin 1,2,ZHENG Yong-lian 2
Abstract:The young embryo was used as explant,we studied the effect of some factors on induce frequency In studied factors,the medium component,embryo size and lay pattern of young embryo were involved Type II callus of maize inbred A188 were obtained,and plantlet were induced from callus too Technological platform of tissue culture in maize inbred A188 was established by our research
Keywords:maize(  Zea mays  L  )  young embryo  callus  tissue culture
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