灰霉病菌抗感腐霉利菌株形态和生理生化特性的比较

经紫外线照射、亚硝基胍处理和药剂诱导,分别从灰霉病菌野生敏感菌株XY6-1S获得高抗腐霉利菌株XY6-1R052、XY6-1R140和XY6-1R202,其EC_(50)>100,μg·mL~(-1)、MIC>1 000μg·mL~(-1).在1～4μg·mL~(-1)药剂浓度下,敏感菌株菌丝呈现不同程度的形态异常,甚至细胞壁破损,而抗性菌株菌丝未有明显改变.在含20μg·L~(-1)以上蔗糖或1.25 g·L~(-1)以上NaCl培养基上,抗药菌株菌丝生长明显差于敏感菌株,表明前者比后者对高渗透压敏感.另外,抗药菌株菌丝相对电导率显著高于敏感菌株,显示抗药菌株细胞膜透性明显改变,因而导致电介质渗漏.在含药培养液中,敏感菌株菌丝甘油含量急剧上升,提高904.63%,而抗药菌株菌丝甘油含量仅增加20.54%～44.00%,因此对于高渗环境,敏感菌株具有较好的调节能力.

Comparison in morphological, physiological and biochemical feathers betweenthe resistant mutants and the sensitive strain of Botrytis cinerea to procymidone

Three resistant mutants XY6-1R052, XY6-1R140, XY6-1R202 to procymidone were obtained from the sensitive wild strain XY6-1S of Botrytis cinerea treated with ultraviolet, N-methyl-N-nitroguanidine and procymidone respectively. They had high resistance with EC_(50) of >100μg·mL~(-1) and MIC of >1 000μg·mL~(-1). Procymidone at the concentration of 1-4μg·mL~(-1) could not only hinder the mycelial growth of the sensitive strain, but also lead to the hyphal distortion and cell wall disruption. But there were no distinct effects on the resistant mutants in the same condition. The mycelial growth of the resistant mutants was significantly worse on the media with sucrose (>20 g·L~(-1)) or NaCl (>1.25 g·L~(-1)) than that of the sensitive strain, which indicated that the resistant strains were more sensitive to high osmotic stress than the sensitive one. In addition, the relative conductivity of the resistant mutants were obviously higher than that of the sensitive strain, which showed that the membrane permeability of the resistant strains was distinctly changed to leak cell electrolyte out. When grown in the media amended with procyrnidone, glycerol the hyphae of the sensitive strain sharply increased and abundantly accumulated by 904. 63％ while that in the resistant mutants only increased by 20.54％-40.00％, which implied that the sensitive strain had better regulation ability to osmotic stress than the resistant mutants.