| 适于AFLP分析的柿树DNA提取方法研究 |
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| 引用本文： | 王文江,张桂霞,张志华. 适于AFLP分析的柿树DNA提取方法研究[J]. 河北农业大学学报, 2004, 27(5): 41-45 |
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| 作者姓名： | 王文江 张桂霞 张志华 |
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| 作者单位： | 河北农业大学,山区研究所,河北,保定,071001;天津农学院,园艺系,天津,300384 |
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| 摘 要： | 对柿基因组DNA的提取方法进行了研究,结果表明,采用提取液中含100mmol/LTris-HCl(pH8 0),20mmol/LEDTA,1 4mol/LNaCl,2%CTAB,1%PVP-40,10mmol/LNa2S2O5及100mmol/Lβ—巯基乙醇(用前加入)的改良CTAB法,可有效去除单宁、酚类、色素等,提取的柿叶片DNA质量和纯度较高,可用于AFLP分析。
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| 关 键 词： | 柿树 DNA提取 AFLP分析 |
| 文章编号： | 1000-1573(2004)05-0041-05 |
Genomic DNA extraction procedure for AFLP analysis in persimmon trees |
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| WANG Wen-jiang,ZHANG Gui-xia,ZHANG Zhi-hua. Genomic DNA extraction procedure for AFLP analysis in persimmon trees[J]. Journal of Agricultural University of Hebei, 2004, 27(5): 41-45 |
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| Authors: | WANG Wen-jiang ZHANG Gui-xia ZHANG Zhi-hua |
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| Affiliation: | WANG Wen-jiang~1,ZHANG Gui-xia~2,ZHANG Zhi-hua~1 |
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| Abstract: | The isolation method of persimmon genomic DNA was studied. The results showed that CTAB(cetyltrimethyl ammonium bromide) buffer supplement with 1% PVP-40 (polyvinylpyrrolidone 40), 100 mmol/L β-mercaptoethanal and 10 mmol/L Na_2S_2O_3 was suitable for genomic DNA extraction of persimmon leaves. Assay of the isolated DNA indicated that its purity was very high, with OD_260/OD_280 value between 1.8 and 2.0. The polyhexose, protein and RNA were eliminated completely, thus the extracted DNA can be used for AFLP analysis. |
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| Keywords: | persimmon genomic DNA extraction AFLP analysis |
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