野生马蹄金种质资源的RAPD鉴别

采用RAPD分子标记技术,从100个随机引物中筛选出多态性强、重复性好且稳定性高的引物22个,对西南区16份抗性较强、坪用价值较高的马蹄金Dichondra repens材料进行扩增,共扩增出269条带,其中多态性带246条,多态性比率为91.45%,表明野生马蹄金植物种间有丰富的遗传多态性。采用UPGMA法聚类分析,在遗传距离0.73处把马蹄金材料分为4类,部分地理上相近的马蹄金种质资源被聚在了不同的大类中,分类结果能将优异马蹄金材料区分开来,与景观价值评定较吻合。

RAPD analysis based identification of Dichondra repens germplasm resources

RAPD molecular markers were used to detect the genetic diversity among 16 materials of Dichondra repens with 22 primers which were selected from 100 random primers. The results indicated that there were 246 polymorphic loci out of totally 269 loci and the polymorphic rate was 91.45%. Genetic distance of D. repens was calculated by Nei method and it ranged from 0.0336 to 0.6097. The RAPD dendrogram suggested that 16 materials of D. repens could be divided into 3 groups at 0.74 of genetic distance, which could screen the excellent materials out and it was similar with the result of landscape evaluation.