| 除臭微生物7NC培养基和培养条件的优化 |
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| 引用本文： | 高红梅,吴东,刘朝良. 除臭微生物7NC培养基和培养条件的优化[J]. 家畜生态, 2011, 0(1): 54-57 |
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| 作者姓名： | 高红梅 吴东 刘朝良 |
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| 作者单位： | 安徽农业大学生命科学学院;安徽省农业科学研究院畜牧兽医研究所; |
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| 基金项目： | 安徽省科技攻关计划面上项目(08010302163); 安徽省自然科学基金资助项目(070411027) |
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| 摘 要： | 针对除臭微生物7NC的应用,采用单因素试验和正交试验的方法对菌株7NC进行了培养基以及培养基组分的筛选。结果表明,7NC较理想的培养基为营养肉汤培养基：蛋白胨10 g/L,氯化钠8 g/L,葡萄糖1 g/L,PH 7.5,酵母粉3 g/L,最适条件为温度37℃,摇床培养（130 r/min）,接种量为3%。试验结果为7NC的大规模生产提供了参数,提高了产量。
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| 关 键 词： | 正交试验 7NC 筛选 |
The Optimization of Medium and Condition for the Deodorization Microorganism 7 NC |
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| GAO Hong-mei,WU Dong,LIU Chao-liang. The Optimization of Medium and Condition for the Deodorization Microorganism 7 NC[J]. Ecology of Domestic Animal, 2011, 0(1): 54-57 |
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| Authors: | GAO Hong-mei WU Dong LIU Chao-liang |
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| Affiliation: | GAO Hong-mei1,WU Dong2,LIU Chao-liang1(1.College of Science,Anhui Agricultural Unitersity.Hefei 230036,China,2.The animal husbandry and veterinary institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China) |
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| Abstract: | For the application of the deodorization microorganism 7NC,use a single factor experiment and orthogonal experiment method for 7NC to screen the culture medium.The results showed that the most suitable culture medium for 7NC was nutrient broth medium： peptone10g/L,Nacl8g/L,glucose1g/L,pH7.5,yeast3g/L,the most suitable conditions was temperature 37℃,shake cultivation（speed 130 r/min）inoculum concentration 3%.The test results provided parameters for large scaled production of 7NC,improved the production. |
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| Keywords: | orthogonal experiment 7NC screen |
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