Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery |
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Authors: | Pal-Bhadra Manika Leibovitch Boris A Gandhi Sumit G Rao Madhusudana Bhadra Utpal Birchler James A Elgin Sarah C R |
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Institution: | Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA. |
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Abstract: | Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. |
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