小油桐外植体分化及植株再生影响因素的初步研究

研究发现在MS培养基中添加6-BA 3 mg.L-1+IBA 0.5 mg.L-1的激素对小油桐苗质及外植体分化有明显促进作用。15 d苗龄的小油桐幼苗外植体有较高的分化率。正交实验研究最佳分化激素浓度,发现6-BA+IBA组合对小油桐外植体芽苗诱导效果明显优于6-BA+NAA组合。子叶再生芽诱导最佳培养基为MS+6-BA 3 mg.L-1+IBA 0.5 mg.L-1,最高诱导率为82.9%;下胚轴再生芽诱导最佳培养基为MS+6-BA 2 mg.L-1+IBA 0.2 mg.L-1诱导率为23.8%。在子叶和下胚轴再生培养基中添加1 mg.L-1的AgNO3能促进小油桐植株再生,并抑制褐化产生。小油桐再生苗最佳生根培养基为WPM培养基。

Studies on influential factors for shoot differentation and plantlet regeneration of Jatropha curcas L.

Studies on the effect for the explant regeneration and the quality of seedlings of Jatropha curcas showed that MS supplied with 6-BA 3 mg·L-1+IBA 0.5 mg·L-1 could obviously promote the explants' differentiation.The explants of 15-day-old seedlings have a higher regenerate rate.Studies on the best hormone density in promoting differentiation,using orthogonal experiment for the tests of 6-BA+IBA and 6-BA+NAA,indicated that the combination of 6-BA and IBA was better than that of 6-BA and NAA.The best cotyledon differentiated medium was MS+6-BA 3 mg·L-1+IBA 0.5 mg·L-1 and the differentiated rate was 82.9%.The best differentiated medium for hypocotyls bud was MS+6-BA 2 mg·L-1+IBA 0.2 mg·L-1,and the differentiate rate was 23.8%.Adding 1 mg·L-1 AgNO3 into the regeneration medium of cotyled and hypocotyls can promote the bud differentiation,and inhibit browning.The WPM medium is the best one in rooting of the regeneration buds.