补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后Bcl-2、Bax表达的影响

目的 探讨补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因Bcl-2、Bax的影响。方法 将SD大鼠80只，雌雄各半，复制局灶性脑缺血大鼠模型，造模后按神经功能缺损评分进行分层，随机分为5组，每组15只，分别于移植后7 d、14 d、28 d进行神经功能评分，采用补阳还五汤超微饮片与传统饮片予以干预，于相应时间点处死大鼠后采用免疫组化法检测凋亡基因Bcl-2、Bax。结果 补阳还五汤能显著减轻神经功能缺损，Bcl-2、Bax在各时间点（7 d、14 d、28 d）表达与模型组比较差异有统计学意义（P<0.05），模型加移植加补阳还五汤超微饮片组上调Bcl-2、拮抗Bax表达的作用优于模型加移植加补阳还五汤传统饮片组，差异有统计学意义（P<0.05）。结论 补阳还五汤可调节脑缺血后Bcl-2、Bax的表达，是其促进神经功能恢复的可能机制之一，且超微饮片组的作用较传统饮片组更加显著。

Effects of Buyang Huanwu Ultra-Fine Powder on Apoptosis Gene Bcl-2 and Bax of Transplanted Neural Stem Cells in Rats with Focal Cerebral Ischemia

Objective To investigate the effects of Buyang Huanwu ultra-fine powder on apoptosis protein of Bcl-2 and Bax in transplanted neural stem cells in rats with focal cerebral ischemia. Methods Eighty Sprague-Dawley rats, half male and half female, were copyed the focal cerebral ischemia models, which were randomly divided into five groups, 15 rats in each group. The model rats were intervened by using Buyang Huanwu ultra-fine powder and traditional decoction. At 7 d, 14 d and 28 d of post-tansport, the neural function defect scale was measured. The rats were sacrified at according time. Bcl-2 and Bax were detected by immunohistochemical staining. Results Buyang Huanwu decoction could improve the neurological defect. Compared with model group, the expression of Bcl-2 and Bax increased obviously at different time points (7 d, 14 d, 28 d), the differnces were statistically significant (P<0.05). The model-transplantation-Buyang Huanwu ultra-fine powder group in increasing Bcl-2 and decreasing Bax was better than the model-transplantation-traditional Buyang Huanwu decoction, the difference was statistically significant (P<0.05). Conclusion Buyang Huanwu decoction could regulate the expression of Bcl-2 and Bax protein after cerebral ischemia, which may be one of mechnisms of promoting the recovery of neural function. The effect of Buyang Huanwu ultra-fine powder was better than traditional decoction.