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小青杨与胡杨杂交及其杂种后代分子鉴定
引用本文:陈洪伟,康向阳,张正海,王君,蔡肖. 小青杨与胡杨杂交及其杂种后代分子鉴定[J]. 北京林业大学学报, 2009, 31(2): 86-91
作者姓名:陈洪伟  康向阳  张正海  王君  蔡肖
作者单位:北京林业大学林木花卉遗传育种教育部重点实验室北京农学院城乡发展学院;北京林业大学林木花卉遗传育种教育部重点实验室;中国农业科学院蔬菜花卉研究所
摘    要:为探讨胡杨与杨属其他派树种远缘杂交的可行性,该研究以小青杨为母本、胡杨为父本进行人工授粉杂交,发现胡杨花粉能够在小青杨雌蕊柱头上萌发,并最终获得了6株杂交后代。杂种幼苗的生长势强弱差异明显,其叶片均为披针形,与父本胡杨幼苗以及萌条的叶形相似; 在SSR标记初步鉴定的基础上,进一步采用AFLP分子标记技术及相应的统计分析,证明父本与母本对子代遗传物质的贡献是等同的,杂交后代是胡杨与小青杨杂交的真杂种。 

关 键 词:胡杨  小青杨  远缘杂交  杂种鉴定
收稿时间:1900-01-01

Production and molecular identification of hybrids between Populus pseudo-simonii Kitag.and P.euphratica Oliv.
CHEN Hong-wei,KANG Xiang-yang,ZHANG Zheng-hai,WANG Jun,CAI Xiao. Production and molecular identification of hybrids between Populus pseudo-simonii Kitag.and P.euphratica Oliv.[J]. Journal of Beijing Forestry University, 2009, 31(2): 86-91
Authors:CHEN Hong-wei  KANG Xiang-yang  ZHANG Zheng-hai  WANG Jun  CAI Xiao
Affiliation:1 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China; 2 Urban and Rural Development College,Beijing University of Agriculture,102206,P.R.China; 3 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081,P. R. China.
Abstract:In order to explore the feasibility of distant hybridization between Populus euphratica Oliv.and other Populus tree species,artificial pollination was performed using P.pseudo-simonii Kitag.as matenal parent and P.euphratica Oliv.as paternal parent.Pollen grains were able to germinate on the stigmas of P.(pseudo-simonii) Kitag.,and six individuals of hybrid were obtained.The growth rate of the hybrid varied significantly among each other.The leaves of all six hybrids were lanceolate,resembling the leaves of...
Keywords:Populus euphratica Oliv.  P.pseudo-simonii Kitag.  distant hybridization  identification of hybrids  
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