花生幼叶丛生芽高效诱导的制约因素研究

以花生品种豫花14号4 d苗龄的幼叶为外植体,对花生幼叶高频不定芽诱导进行研究。在MS培养基的基础上,添加了芽诱导常用的细胞分裂素6-BA、生长素NAA及两种不太常用的脱落酸(ABA)、噻重氮苯基脲(TDZ),结果表明,单独使用6-BA,NAA或TDZ,不定芽诱导率较低;采用较高浓度的6-BA(8 mg/L)、较低浓度的NAA(1 mg/L),添加低浓度的ABA(1 mg/L),不定芽诱导率可达80%以上。最佳诱导培养基为MS+6-BA 8 mg/L+NAA 1 mg/L+ABA 1 mg/L+AgNO32 mg/L,从整体上观察,最佳继代培养基为MS+6-BA 5 mg/L+NAA 2 mg/L+AgNO32 mg/L。同时研究发现,花生幼叶近叶柄基部切口处不定芽诱导率较高,是较理想的不定芽诱导部位。

The Restrictive Factors for Efficient Induction of Adventitious Buds from Peanut Leaflet in vitro

The efficiency of adventitious bud induction from leaflet were investigated using the 4-day seedlings of peanut variety Yuhua 14.The MS medium was added with commonly-used kinin 6-BA,kinetin NAA,and uncommonly-used ABA and thiadiazole Diazo phenyl urea TDZ.Results showed that the adventitious bud induction rate was low in the MS added with only 6-BA,NAA and TDZ,but reached to 80% in the MS added with high concentrations of 6-BA(8mg/L),low concentrations of NAA(1mg/L) and low concentrations ABA(1mg/L),indicating that the MS medium 8mg/L 6-BA 1mg/L NAA 1mg/L ABA 2mg/L AgNO3 had the best result for inducing the adventitious buds.The best medium for subculture was MS 5mg/L 6-BA 2mg/L NAA 2mg/L AgNO3.It was also observed that the underside cut near the petiole presented a higher rate of adventitious buds,so was the ideological explant for adventitious bud induction.