| 风蜡花‘Snow Flake’外植体消毒方法的研究 |
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| 引用本文： | 孟会,李青,潘会堂.风蜡花‘Snow Flake’外植体消毒方法的研究[J].中国农学通报,2010,26(23):271-274. |
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| 作者姓名： | 孟会 李青 潘会堂 |
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| 作者单位： | 北京林业大学园林学院国家花卉工程技术中心,北京100083 |
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| 基金项目： | 国家林业局"948"项目 |
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| 摘 要： | 本文研究了风蜡花‘Snow Flake’组织培养快速繁殖中，外植体消毒的影响因素，分别从灭菌剂及灭菌时间，外植体取材时间、取材部位，以及抗菌素几个方面进行研究。结果表明：在10月到翌年3月取材，以顶芽为外植体，在75%的酒精中浸泡30s后用0.1% HgCl2处理8min，消毒效果较好。而在培养基中添加抗菌素，对‘Snow Flake’ 内生菌污染控制效果不理想。
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| 关 键 词： | 澳蜡花 外植体 组织培养 污染率 |
| 收稿时间： | 2010/4/26 0:00:00 |
| 修稿时间： | 2010/5/24 0:00:00 |
Study on protocol for explant sterilization of Chamelaucium uncinatum in tissue culture |
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| Meng Hui,Li Qing,Pan Huitang.Study on protocol for explant sterilization of Chamelaucium uncinatum in tissue culture[J].Chinese Agricultural Science Bulletin,2010,26(23):271-274. |
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| Authors: | Meng Hui Li Qing Pan Huitang |
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| Institution: | (College of Landscape Architecture,Beijing Forestry University,National Floriculture Engineering Research Center,Beijing 100083) |
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| Abstract: | The effect of explant sterilization of Chamelaucium uncinatum＇ Snow Flake ＇ in tissue culture was studied,such as disinfectors,the time of fetching material,cutting sites and antibiotics,to obtain the optimum method of explant sterilization.Result showed that,the optimum explants were terminal bud cut from October to March in next year,and the optimum way of sterilizing treatment was that explants were dipped in 75% alcohol for 30s,then in 0.1% HgCl 2 for 8 min.However antibiotics added into media can not inhibit contamination caused by endophytes.Therefore,explant sterilization of Chamelaucium uncinatum＇ Snow Flake ＇ is related to disinfectors,the time of fetching material and cutting sites. |
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| Keywords: | Chamelaucium uncinatum explant tissue culture contamination |
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