Specific binding of dl-cloprostenol and d-cloprostenol to PG F2α receptors in bovine corpus luteum and myometrial cell membranes |
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Authors: | G RE P BADINO A NOVELLI A VALLISNERI C GIRARDI |
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Institution: | *Department of Animal Pathology, Division of Pharmacology &Toxicology, University of Turin, Turin, Italy;?FATR0 s.p.a. Ozzano Emilia, Bologna, ltaly |
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Abstract: | Prostaglandin F2α receptors (PGF2α Rs) were measured in bovine corpus luteum and myometrial cell membranes using a radiometric method. The inhibition of labelled PGF2α binding exerted by d-cloprostenol, dl-cloprostenol, PGF2α and PGE1 (l0–11 M to 10–4 M) was evaluated in vitro. Results strongly suggest that cloprostenol binding to PGF2αRs is stereospecific. d-Cloprostenol and PGF2α were equipotent, about 150 times more potent than d-cloprostenol (P < 0.05) and approximately 280 times more potent than PGE, (P < 0.05) in inhibiting 3H]PGF2α binding to corpus luteum cell membranes. Such differences were less evident in myometrial cell membranes, where d-cloprostenol and PGF2α were about 10 times more potent than d-cloprostenol (P < 0.05) and approximately 95 times more potent than PGEl (P < 0.05). |
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