Regional distribution of microsomal xenobiotic and steroid metabolism in kidney microsomes from rainbow trout |
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Authors: | Maija Pesonen Tiiu Hansson Lars Förlin Tommy Andersson |
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Affiliation: | 1. Department of Zoophysiology, University of G?teborg, Box 25059, S 400 31, G?teborg, Sweden 2. Department of Medical Nutrition, Karolinska Institution, Hudding Hospital, S-149 86, Huddinge, Sweden
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Abstract: | The regional distribution of microsomal cytochrome P-450-mediated reactions with exogenous and endogenous substrates in the kidney of rainbow trout was studied. The cytochrome P-450-dependent 7-ethoxyresorufin- and 7-ethoxycoumarin-0-deethylase activities were significantly higher (3–4 and 10–14 fold, respectively) in the trunk kidney than in the head kidney, whereas ethylmorphine-N-demethylase activity was evenly distributed along the kidney. The microsomal cytochrome P-450-dependent steroid hydroxylases and steroid reducing enzymes when using androstenedione as substrate also exhibited a regional distribution in trout kidney. The 6β- and 16-hydroxylase activities as well as the 5α-reductase and 17 hydroxysteroid oxidoreductase activities were higher in the anterior part of the trunk kidney than in the head kidney and posterior trunk kidney. |
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Keywords: | fish rainbow trout Salmo gairdneri kidney cytochrome P-450 metabolism xenobiotics steroids |
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