灵芝子实体中灵芝酸A的HPLC定量测定方法

为建立一种灵芝子实体中灵芝酸A的高效液相色谱（HPLC）定量测定方法．通过对灵芝子实体萃取物分离效果的影响因素进行对比试验．确定了灵芝中灵芝酸A的分离条件：色谱柱为ZORBAXSB—C18（4．6mm×150mm，5μm）；流动相组成（A：1．0％醋酸水溶液，B：甲醇），A：B比例为40％：60％，流速0．5mL／min，柱温25℃，进样量5μL；在此条件下，灵芝子实体萃取物中灵芝酸A的HPLC保留时间13．619min，分离度〉1．5；绘制的标准曲线为y=8．8021x-1．45，r^2=0．9902；其测定结果的重现性、精密度和加标回收率（99．58％）均较佳，RSD均小于2％。因此，该方法可作为灵芝子实体中灵芝酸A的HPLC定量测定方法。

Study on Quantitative Determination Method of Ganoderic Acid A from Ganoderma lucidum by HPLC

In order to establish a quantitative measurement method to dertermine the ganoderic acid A from Ganoderma lucidum by HPLC, the comparative experiments were used to study the effects of several influencing factors on separation of ganoderie acid A and the separation condition was determined. The results showed that, the optimum HPLC separation was accomplished through ZORBAX SB-C18 column （4.6 mm×lS0 mm, 5 μm）, taking 1.0% acetate buffer and methanol （40% ： 60%） as mobile phase, 0.5 mL/min as flow rate, 25 ℃ as column temperature and 5 μL as injection volume. Under the HPLC separation condition, the retention time of the ganoderic acid A was 13.619 min and the resolution was larger than 1.5. The standard curve was y=8.802 lx-1.45（r^2=0.990 2）. The repeatability, precision and recovery （99.58%） of the quantitative measurement method were all good, and relative standard deviation （RSD） of all results were less than 2%. Therefore, the obtained HPLC method could be used as the quantitative determination method of ganoderic acid A from Ganoderma lucidum.