| ICE1基因表达载体的构建及对番茄的转化 |
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| 引用本文： | 张玉,蒋欣梅,于锡宏. ICE1基因表达载体的构建及对番茄的转化[J]. 中国蔬菜, 2010, 1(18): 27-33 |
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| 作者姓名： | 张玉 蒋欣梅 于锡宏 |
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| 作者单位： | 东北农业大学园艺学院蔬菜生理与设施园艺研究室，黑龙江哈尔滨 150030 |
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| 基金项目： | 黑龙江省教育厅项目，哈尔滨市科技创新人才研究专项资金项目，东北农业大学创新团队课题，黑龙江省教育厅寒地蔬菜生物学重点实验室 |
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| 摘 要： | 以pCAMBIA3301质粒为基础,构建了由CaMV35S启动子调控的ICE1基因植物表达载体p3301-ICE1。重组质粒通过农杆菌介导转化番茄品种组培大黄经卡那抗性鉴定,获得5株含ICE1基因的番茄抗性植株,PCR扩增和RT-PCR检测结果表明,其中3株为阳性植株,转化率为60%。转ICE1基因的番茄植株经4℃低温胁迫72h后,与对照植株相比,MDA含量明显降低,Pro含量、POD和CAT活性明显升高。
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| 关 键 词： | ICE1基因 植物表达载体 番茄 |
| 收稿时间： | 2010-03-07; |
Construction of ICE1 Gene Expression Vector and Transformation into Tomato (Lycopersicon esculentum Mill.) |
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| ZHANG Yu,JIANG Xin-mei,YU Xi-hong. Construction of ICE1 Gene Expression Vector and Transformation into Tomato (Lycopersicon esculentum Mill.)[J]. China Vegetables, 2010, 1(18): 27-33 |
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| Authors: | ZHANG Yu JIANG Xin-mei YU Xi-hong |
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| Affiliation: | Vegetable Physiology and Installation Horticulture Laboratory，College of Horticulture，Northeast Agricultural University，Harbin 150030，China |
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| Abstract: | This experiment was based on the plant expression vectorPCAMBIA3301，constructed the plant expressing vectors P3301-ICE1，Carrying an Arabidopsis thaliana ICE1 gene under the drive of CaMV35S promoter.the plasmid was transferred into tomato（Lycopersicon esculentum Mill）“cultivar Da Huang”via agrobacterium-mediated transformation.5 cultivar Da Huang with Kan-resistance were obtained，The results of test PCR amplifications of ICE1 gene and RT-PCR proved that obtain 3 positive plant of cultivar‘Da Huang’，positive plant rate was 60%.The transgenic tomato were treated with low temperature（4℃）stress for 72h，the content of MDA were apparently lowered，the content of Pro and the activityof CAT and POD were significant higher.compared with non-transgenic tomato. |
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| Keywords: | plant expression vector tomato |
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