单根阴离子交换柱法提取L-谷氨酰胺工艺研究

目的]研究不同影响因素及工艺条件对提取L-谷氨酰胺的影响，为谷氨酰胺的工业化生产提供一种新型分离工艺。方法]利用单根阴离子交换柱，采用静态吸附试验和动态分离试验研究从酶法合成液中提取谷氨酰胺的各种条件。结果]模拟液离交分析的最适条件为：树脂201x4，进样流速为3．0BV／h，进样pH值6．5，谷氨酸浓度1．O％。酶反应液处理的工艺条件为：脱色温度40℃，pH值6．5，10．0g/L粉末状活性炭进行二次脱色，15．0mmol／L的Ca2＋添加量来去除样液中PO4^3＋，离交分离条件同模拟液。Gin收率为66．6％、Glu去除率为95．O％。结论]该研究得到的处理方法简单易行，Gin收率和Glu去除率高，在工业生产中有较好的应用前景。

Research on the Extraction Technology of L-glutamine with A Single Anion Exchange Column

Objective] The new type of isolation process for the industrial production of glutamine was provided through the study on the different factors processing conditions impacting the extraction of L-glutamine.Method] The conditions of glutamine extracting from the solution of enzymatic synthesis were researched in a single anion-exchange column with static absorption and dynamic separation method.Results] The result indicated that the optimal conditions simulated sample liquid were as follows: the resin,201...