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Analysis of tissue culture-derived variation in pea (Pisum sativum L.)-preliminary results
Authors:Miroslav Griga  Jaromír Stejskal and Karel Beber
Institution:(1) OSEVA, Research Institute of Technical Crops & Legumes, 787 01 Scaronumperk, Czech Republic;(2) Research Institute for Cattle Breeding, Rapotín 267, 787 13 Vikýrcaronovice, Czech Republic
Abstract:Summary The possibility of producing agronomically-useful somaclones via organogenesis and somatic embryogenesis from callus cultures of pea (Pisum sativum L.) was studied. Organogenic calli were induced from immature leaflets on MSB medium with NAA and BAP. Embryogenic calli were derived either from immature zygotic embryos (using 2,4-D) or from shoot apices (using picloram) of aseptically-germinated seedlings.The seed progenies (T1 to T3-generation) of primary regenerants were grown in field conditions and their phenotypic variation was evaluated and compared with control, non-tissue culture-derived plant material. In addition, electrophoretic analyses of selected isoenzyme systems and total proteins have been done. The results do not show dramatic changes in qualitative and quantitative traits. The evaluation of at least two future generations (T4, T5) is planned.Abbreviations BAP 6-benzylaminopurine - IBA indole-3-butyric acid - MSB medium (mineral salts after Murashige & Skoog, 1962, vitamins after Gamborg et al., 1968) - NAA agr-naphthalene-acetic acid, picloram-4-amino-3,5,6-trichloro picolinic acid - 2,4-D 2,4-dichlorophenoxyacetic acid - ORG organogenesis - SE somatic embryogenesis
Keywords:callus culture  organogenesis  pea  Pisum sativum  somaclonal variation  somatic embryogenesis
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