| 应用SSR标记技术鉴定强盛16号玉米单交种纯度 |
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| 引用本文： | 武岩军,许晶,车星星. 应用SSR标记技术鉴定强盛16号玉米单交种纯度[J]. 山西农业科学, 2012, 40(6): 599-602 |
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| 作者姓名： | 武岩军 许晶 车星星 |
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| 作者单位： | 1. 山西省农业科学院试验研究中心,山西太原,030006
2. 山西省农业科学院食用菌研究所,山西太原,030031 |
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| 基金项目： | 山西省农业科学院科技攻关项目 |
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| 摘 要： | 试验以强盛16号玉米单交种及相应亲本自交系和20对玉米SSR位点引物为材料,筛选出了适合该杂交种纯度鉴定的SSR位点引物。结果表明,bnlg2331引物的扩增产物呈现双亲互补的特征谱带,易于区分杂交种和亲本自交系。并通过对单籽粒DNA提取等技术环节的改进,建立了一套简单、快速、准确、可靠的种子纯度鉴定方法。
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| 关 键 词： | 玉米杂交种 DNA提取 SSR标记技术 种子纯度鉴定 引物筛选 |
Application of SSR Marker Technique to Purity Testing of Maize Hybrid Qiangsheng 16 |
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| WU Yan-jun , XU Jing , CHE Xing-xing. Application of SSR Marker Technique to Purity Testing of Maize Hybrid Qiangsheng 16[J]. Journal of Shanxi Agricultural Sciences, 2012, 40(6): 599-602 |
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| Authors: | WU Yan-jun XU Jing CHE Xing-xing |
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| Affiliation: | 1.Research Center of Experiment,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China; 2.Institute of Edible Fungi,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China) |
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| Abstract: | In this study,20 SSR primer pairs were screened to search for the SSR loci that were suitable for testing the purity of maize hybrid Qiangsheng 16,which lacked a reliable laboratory method for purity testing.The results were as follows:a polymorphic SSR loci bnlg2331 was found that could be applied to test the purity of this hybrid.A procedure featuring inexpensive equipment and easy use were set up for testing maize hybrid purity using SSR marker. |
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| Keywords: | maize hybrid DNA extraction SSR marker technique seed purity testing primer pairs screening |
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