| 几种检测弓形虫血清抗体方法的比较分析 |
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| 引用本文： | 刘帅,王贝贝,崔丽丽,靳兴军,张跃,沈光年,汪明. 几种检测弓形虫血清抗体方法的比较分析[J]. 中国兽医杂志, 2012, 48(2): 72-74 |
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| 作者姓名： | 刘帅 王贝贝 崔丽丽 靳兴军 张跃 沈光年 汪明 |
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| 作者单位： | 1. 中国农业大学动物医学院国家动物原虫实验室,北京海淀,100193
2. 北京市兽医实验诊断所,北京朝阳,100101 |
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| 基金项目： | 公益性行业(农业)科研专项(200903036-03) |
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| 摘 要： | 本文对5种弓形虫血清IgG抗体检测方法进行比较评价。5种方法平行检测了80份血清,比较各检测方法的阳性检出率,敏感性,特异性,符合率及Youden指数。结果经配对2检验,ELISA-200,IHA,MAT,SPA-ELISA 4种方法在阳性检出率、平行检测结果之间无显著性差异(P<0.05)。ELISA-200敏感性、特异性和符合率均高于90%,Youden指数(0.85)显著高于其他方法(P<0.05)。IHA,MAT,SPA-ELISA 3种方法各指标处于中等水平,各有适合的检测环境。ELISA-100与其他四种方法在检测结果上差异极显著(P<0.01),特异性(38.3%)、符合率(58.75%)及Youden指数(0.383)都显著低于其他方法。
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| 关 键 词： | 弓形虫 ELISA IHA MAT |
Comparison of several methods for detection of IgG against Toxoplasma gondii |
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| LIU Shuai,WANG Bei-bei,CUI Li-li,JIN Xing-jun,ZHANG Yue,WANG Ming. Comparison of several methods for detection of IgG against Toxoplasma gondii[J]. Chinese Journal of Veterinary Medicine, 2012, 48(2): 72-74 |
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| Authors: | LIU Shuai WANG Bei-bei CUI Li-li JIN Xing-jun ZHANG Yue WANG Ming |
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| Affiliation: | 1(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China; 2.Veterinary Diagnosis Laboratory of Beijing,Beijing 100101,China) |
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| Abstract: | To evaluate five kits for the detection of serum IgG antibody against Toxoplasma gondii.A total of 80 pig serum samples were tested by those 5 kits.The five kits were compared for the positive rate,sensitivity,specificity,efficiency,and Youden index.The results showed that there were no significant differences among IHA,MAT,SPA-ELISA,ELISA-200 parallel detection results.ELISA-200 had the best efficiency(93.75%),and the Youden index of ELISA-200 was significantly higher than the others(P<0.05),suggesting it has the highest diagnosis value.IHA,MAT,SPA-ELISA had lower efficiency.The result of ELISA-100 is significantly different from the other four methods with lower specificity,efficiency and Youden index,indicating that the kit has low-value for diagnosis. |
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| Keywords: | toxoplasma ELISA IHA MAT |
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