| 马铃薯块茎组织特异性启动子GBSS的克隆及序列分析 |
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| 引用本文： | 陈国梁,陈宗礼,齐向英,等.马铃薯块茎组织特异性启动子GBSS的克隆及序列分析[J].长江蔬菜,2011(8):25-27. |
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| 作者姓名： | 陈国梁 陈宗礼 齐向英 等 |
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| 作者单位： | 陕西省延安大学生命科学学院/陕西省区域生物资源保育与利用工程技术研究中心; |
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| 基金项目： | 陕西省教育厅省级重点实验室重点科研计划项目(2010JS065); 延安市科技发展计划项目(2010kn-03); 延安大学专项科研基金项目、延安大学大学生科技创新训练项目(YD2008-106) |
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| 摘 要： | 利用PCR技术从马铃薯陇薯3号基因组DNA中扩增出长度约为600 bp的DNA片段,经与T载体连接,测序表明,克隆到的DNA片段大小为599 bp,该序列与Genebank中已公布的GBSS启动子序列同源性为99.67%;采用植物顺式调控元件数据库PLACE和PlantCare对其进行序列分析,结果表明,该片段含有启动子的保守序列TATA-box和CAAT-box。此外,还具有诸如TAACAAA、CTAACAC、CTCTT及CACT等序列,而这些特异序列可能是基因特异表达所必须的。
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| 关 键 词： | 马铃薯 组织特异性 启动子 PCR |
Cloning and Sequencing the GBSS of Potato Tuber Tissue-specific Promoter Region |
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| CHEN Guoliang,CHEN Zongli,QI Xiangying,HE Xiaolong.Cloning and Sequencing the GBSS of Potato Tuber Tissue-specific Promoter Region[J].Journal of Changjiang Vegetables,2011(8):25-27. |
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| Authors: | CHEN Guoliang CHEN Zongli QI Xiangying HE Xiaolong |
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| Institution: | CHEN Guoliang,CHEN Zongli,QI Xiangying,HE Xiaolong(College of Life Science,Yan'an University/Shanxi Engineering & Technological Research Center for Conversation & Utilization of Regional Biological Resources,Yan'an,Shanxi 716000) |
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| Abstract: | The GBSS gene promoter region was amplified from solanum tuberosumn genomic DNA by polymerase chain reaction(PCR) and cloned into the T-vector.The sequence analysis showed that the sequence size was 599 bp,its homology was 99.67% with published sequence of the GBSS promoter in Genebank.Using PLACE and PlantCare sequence database analysis showed that the fragment containing the conserved promoter sequence,such as TATA-box,CAAT-box.In addition,the fragment containing TAACAAA,CTAACAC,CTCTT and CACT specific ge... |
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| Keywords: | Potato Tissue-specific Promoter PCR |
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