| 茉莉花愈伤组织诱导及悬浮细胞培养 |
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| 引用本文： | 庄哲煌,蔡汉权,陈丹生.茉莉花愈伤组织诱导及悬浮细胞培养[J].长江蔬菜,2011(8):21-24. |
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| 作者姓名： | 庄哲煌 蔡汉权 陈丹生 |
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| 作者单位： | 韩山师范学院生物系; |
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| 基金项目： | 韩山师范学院重点科研项目(2005-413545) |
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| 摘 要： | 以茉莉花（Jasminum sambac）花瓣为外植体在不同培养基上诱导出愈伤组织,通过对其类型的筛选从中选择疏松愈伤组织进行悬浮培养后,初步建立悬浮细胞培养系。结果表明,不同激素可诱导出疏松型（CA）、泥状型（CB）和绿色块状型（CC）愈伤组织,其分化方向也不同。用CA型和CB型愈伤组织均能建立悬浮细胞培养系,其中以泥状愈伤组织为起始材料较佳,大约11 d可建立初步的悬浮细胞培养系。
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| 关 键 词： | 茉莉花 愈伤组织 悬浮细胞培养 |
Jasminum sambac Callus Induction and Suspension Cell Culture |
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| ZHUANG Zhehuang,CAI Hanquan,CHEN Dansheng.Jasminum sambac Callus Induction and Suspension Cell Culture[J].Journal of Changjiang Vegetables,2011(8):21-24. |
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| Authors: | ZHUANG Zhehuang CAI Hanquan CHEN Dansheng |
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| Institution: | ZHUANG Zhehuang,CAI Hanquan,CHEN Dansheng(Department of Biology,Hanshan Normal University,Chaozhou,Guangdong 521041) |
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| Abstract: | Petals of Jasminum sambac were induced and cultured callus on the media with different phytohormones concentration.The preliminary stable suspension culture system was established after choosing the loose callus from different types for cell suspension culture.The results indicated that different phytohormones could form three types of callus,CA,CB and CC which had diverse polarization direction.Both the CA and CB callus could be used as initial materials for establishing suspensions,and it took about 11 da... |
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| Keywords: | Jasminum sambac Callus Cell suspension culture |
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