| 萝卜与大头菜属间杂种胚的离体培养 |
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| 引用本文： | 甘彩霞,何云启,崔磊,等. 萝卜与大头菜属间杂种胚的离体培养[J]. 长江蔬菜, 2011, 0(8): 18-20. DOI: 10.3865/j.issn.1001-3547.2011.08.006 |
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| 作者姓名： | 甘彩霞 何云启 崔磊 等 |
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| 作者单位： | 湖北省农科院经济作物所; |
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| 基金项目： | 国家科技支撑子课题十字花科及特种蔬菜优异基因源挖掘与种质创新利用研究(2006BAD13B06-4-4) |
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| 摘 要： | 用萝卜雄性不育系DH303A作母本,以大头菜为父本进行属间远缘杂交,杂交后代的初期结荚率为64%~88%,横径第7天停止增加,荚长第8天停止生长,授粉以后第7天开始败育,而且越往后败育得越快。在杂种胚发育后5～8 d进行胚抢救,用生芽培养基（1/2 MS＋1.0 mg/L 6-BA＋0.2 mg/L NAA＋0.5%活性炭）和生根培养基（1/2 MS＋0.2 mg/L 6-BA＋1.0 mg/L NAA＋0.5%活性炭）进行离体培养,能成功获得幼苗。
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| 关 键 词： | 萝卜 大头菜 远缘杂交 胚抢救 离体培养 |
Intergeneric Hybrid Embryo in Vitro Culture of Raphanus sativus L. and Brassica juncea Coss. var. napiformmis Pal et. Bols |
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| GAN Caixia,HE Yunqi,CUI Lei,MEI Xuefei,MEI Shiyong. Intergeneric Hybrid Embryo in Vitro Culture of Raphanus sativus L. and Brassica juncea Coss. var. napiformmis Pal et. Bols[J]. Journal of Changjiang Vegetables, 2011, 0(8): 18-20. DOI: 10.3865/j.issn.1001-3547.2011.08.006 |
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| Authors: | GAN Caixia HE Yunqi CUI Lei MEI Xuefei MEI Shiyong |
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| Affiliation: | GAN Caixia,HE Yunqi,CUI Lei,MEI Xuefei,MEI Shiyong(Hubei Provincial Academy of Agricultural Crops,Wuhan 430072) |
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| Abstract: | A male sterile line DH303A of Raphanus sativus L.as the female parent,crossed with a male line of Brassica juncea Coss.var.napiformmis Pal et.Bols.This was an intergeneric hybrid and their embryo had been developed in medium(1/2MS + 1.0 mg/L 6-BA + 0.2 mg/L NAA + 0.5% activated carbon) and the rooting medium(1/2MS + 0.2 mg/L 6-BA +1.0 mg/L NAA + 0.5% activated carbon) after the 5-7 days during budding.Seedlings could be obtained successfully through this procedure. |
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| Keywords: | Raphanus sativus L. Brassica juncea Coss.var.napiformmis Pal et.Bols Distant hybirdization Embryo rescue Hybrid embryos Culture in vitro |
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