生防菌HT3和HT5代谢产物对灰葡萄孢菌的抑菌作用

为了明确从核桃上分离筛选的2株内生菌HT3和HT5的代谢产物对灰葡萄孢菌的抑菌活性及其与农药混配后的抑菌效果,采用孢子萌发法和菌丝生长速率法进行了室内抑菌作用测定。结果表明,菌株HT3和HT5代谢产物对灰葡萄孢菌孢子萌发影响较大,致使孢子畸形或抑制萌发,抑制率分别达到100%和98%;菌株HT3和HT5代谢产物对灰葡萄孢菌菌丝生长也均有显著的抑制效果,其中,菌株HT3代谢产物的抑制率为58.69%,菌株HT5代谢产物的抑制率为63.07%。HT3,HT5代谢产物与杀菌剂速克灵50倍稀释液混配对病原菌菌丝生长的抑制具有一定的增效作用,其中,以速克灵(50倍稀释液)∶HT3=50∶50,速克灵(50倍稀释液)∶HT5=25∶75这2个组合的抑制率较高,分别为87%和86%,增效比均为1.3。

Antagonism of Metabolites from Biocontrol Bacteria HT3 and HT5 on Botrytis cinerea

HT3 and HT5 are endophytic bacterium isolated from walnut. To determin their control effect on Botrytis cinerea, we tested the bacteria inhibitive rate on Botrytis cinerea with the mycelium growth rate and spore germination method. The results showed that the metabolites of HT3 and HT5 had significantly inhibited the spores germination, The metabolites of HT3 had strong inhibiting effect on Botrytis cinerea and spore germination inhibition rate was 100%; the metabolites of HT5 led to spores germination deformity and the inhibition rate was 98%. And their metabolites had significantly inhibitory effect on Botrytis cinerea, antibacterial rates 58.69% and 63.07% respectively.The mycelial growth inhibition activity of HT3 and HT5 bacterial metabolites mixed with 50% procymidone WP had a synergistic effect, in which the inhibition rate of procymidone ： HT3 = 50 ： 50,and procymidone： HT5 = 25 ： 75 were the highest, 87% and 86% respectively, and both synergism ratios were 1.3.