| 全氟辛烷磺酸酶联免疫检测方法的研究 |
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| 引用本文： | 朱江,杨道丽,李炳智,陈唯尔,宋姗姗.全氟辛烷磺酸酶联免疫检测方法的研究[J].上海交通大学学报(农业科学版),2014(2):74-78. |
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| 作者姓名： | 朱江 杨道丽 李炳智 陈唯尔 宋姗姗 |
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| 作者单位： | [1]上海市环境科学研究院固体废物与土壤环境研究所,上海200233 [2]昆明理工大学环境科学与工程学院,云南650118 |
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| 基金项目： | 上海市自然科学基金项目（12ZR1426500）;上海市自然科学基金项目（13ZR1435200）;上海市技术标准专项项目（10DZ0503900）;国家自然科学基金项目（21047001） |
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| 摘 要： | 以人工合成的全氟辛烷磺酸免疫原免疫新西兰大白兔,制备多克隆抗体,用辛酸-硫酸铵沉淀法纯化抗体,选择包被抗原浓度为0.1μg/mL,抗体稀释度为1∶8 000,建立酶联免疫(ELISA)检测方法。交叉反应试验结果表明,除全氟癸烷磺酸和全氟己烷磺酸的交叉反应率偏高(16.2%,14.22%)外,与其他全氟化合物交叉反应率低于10%,即该抗体有较好的特异性。PFOS检测限为10μg/L,该ELISA方法的最佳工作范围在10~1 000μg/L之间,在此范围内平均回收率为101.62%,平均变异系数为2.4%,表明该方法准确度和精密度高,便于在线检测,可应用于对水体中全氟辛烷磺酸残留的酶联免疫检测。
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| 关 键 词： | 酶联免疫吸附测定法 全氟辛烷磺酸 交叉反应 水样分析 |
| 收稿时间： | 2012/6/7 0:00:00 |
Development of an Indirect Competitive ELISA for the Detection of the Perfluorooctane Sulphonate |
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| ZHU Jiang,YANG Dao-li,LI Bing-zhi,CHEN Wei-er and SONG Shan-shan.Development of an Indirect Competitive ELISA for the Detection of the Perfluorooctane Sulphonate[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(2):74-78. |
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| Authors: | ZHU Jiang YANG Dao-li LI Bing-zhi CHEN Wei-er and SONG Shan-shan |
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| Institution: | (Institute of Wastes and Soil Remediation, Shanghai Academy of Environmental Sciences, Shanghai 200233,China;School of Environmental Science and Engineering, Kunming University of Science and Technology, Yunnan 650118,China) |
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| Abstract: | To develope an indirect competitive enzyme-linked immunosorbent assay （ELISA） for detecting PFOS,the polyclonal antibodies against PFOS （hapten） was prepared by immunizing New Zealand white rabbits with PFOS immunogen.The antibody was purified by caprylic acid-ammonium sulfate precipitation method,the coating antigen concentration and antibody dilution were 0.1μg/mL and 1∶8 000,respectively.The results showed that the quantitative working range of the ELISA for determining PFOS was from 10 to 1 000μg/L with a limit of detection of 10 μg/L.Cross-reactivity to structurally similar PFCs and related chemicals were determined.The antiserum had cross-reactivity with PFDS （16.2％） and PFHxS （14.22 ％）,but had relatively low cross-reactivity with other PFCs （＜ 10％）.Four kinds of water samples were fortified with PFOS at several concentration levels and were directly analyzed.The mean recovery and coefficient of variation were 101.62％ and 2.4％,respectively.The proposed ELISA for detecting PFOS hopfully can be a powerful tool for monitoring of residual PFOS in water samples at trace level. |
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| Keywords: | ELISA PFOS cross-reactivity water analysis |
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