| 基于汉赛巴尔通体外膜蛋白构建ELISA检测方法及其应用 |
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| 引用本文： | 郁达义,白雅洁,洪杰华,张敏,顾永远,袁聪俐. 基于汉赛巴尔通体外膜蛋白构建ELISA检测方法及其应用[J]. 上海交通大学学报(农业科学版), 2014, 0(2): 1-4, 14 |
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| 作者姓名： | 郁达义 白雅洁 洪杰华 张敏 顾永远 袁聪俐 |
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| 作者单位： | [1]上海市闵行区动物疾病预防控制中心,上海20110 [2]上海交通大学农业与生物学院,上海200240 |
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| 基金项目： | 上海市自然科学基金（12ZR1414000）;2012博士点新教师基金（20120073120068）;2012闵行区自然科学基金（2012MHZ007） |
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| 摘 要： | 汉赛巴尔通体是人类猫抓病的主要病原体,亦是艾滋病人及部分免疫低下人群杆菌性血管瘤的致病原。本实验利用汉赛巴尔通体外膜蛋白建立ELISA检测方法。通过平行检测实验证实本研究建立的ELISA方法检测效率与商品化免疫荧光(IFA)试剂盒具有较高一致性。运用本研究建立的ELISA方法初步对180个健康志愿者的血液样本和34个淋巴结病患者血液样本进行检测,结果发现25%的健康志愿者,41%的患者血清反应呈巴尔通体阳性感染。
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| 关 键 词： | 汉赛巴尔通体 外膜蛋白 ELISA |
| 收稿时间： | 2013-03-02 |
Establishment of a Home-Made ELISA Method Based on the Outer Membrane Protein of Bartonella henselae |
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| YU Da-yi,BAI Ya-jie,HONG Jie-hu,ZHANG Ming,GU Yong-yuan and YUAN Cong-li. Establishment of a Home-Made ELISA Method Based on the Outer Membrane Protein of Bartonella henselae[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2014, 0(2): 1-4, 14 |
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| Authors: | YU Da-yi BAI Ya-jie HONG Jie-hu ZHANG Ming GU Yong-yuan YUAN Cong-li |
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| Affiliation: | (Shanghai Minhang Center for Disease Control and Prevention, Shanghai 201109, China;School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | A home-made ELISA kit for detection of Bartonella henselae （Bh） infection in humans was established by using outer membrane proteins.Efficacy of the established ELISA was convinced bycomparing with commercial IFA kit.180 blood samples from healthy volunteers and 43 blood samples from inpatients with lymphadenitis were tested by the method of ELISA.The results showed that 25％ out of the180 healthy volunteers and 41％ out of the 34 patients were seropositive with Bh infection. |
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| Keywords: | Bartonella henselae outer membrane proteins ELISA |
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