A shoot induction procedure altered for increased shooting efficiency of potato protoplast-derived calli (cvs. crystal and norgold russet) |
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Authors: | R. J. Taylor G. A. Secor |
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Affiliation: | (1) Department of Plant Pathology, North Dakota State University, 58105 Fargo, ND, USA |
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Abstract: | Summary The responses of two potato cultivars, Crystal and Norgold Russet, to a standard protoplast regeneration procedure were examined. Both cultivars yielded protoplasts that survived and grew in the initial plating medium. Protoplast-derived callus tissue also grew on the callus proliferation medium. The cultivars differed most in their ability to survive on the shoot induction medium; changes in its growth regulator content and the quantity of agar increased shoot production of Crystal from 1% to 30% and that of Norgold Russet from 7% to 24% on modified medium under low light intensity. Journal Series Paper 1699 of the North Dakota Agricultural Experiment Station. |
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Keywords: | callus culture regeneration Solanum tuberosum L |
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