植物细胞生长周期中内外源细胞激动素的变化

应用细胞同步培养技术、高压液相色谱分析（ＨＰＬＣ）和酶偶联免疫法（ＥＬＩＳＡ）分析了胡萝卜细胞在悬浮培养时生长分裂周期的变化并对内源激动素类物质（玉米素Ｚ－Ｚｅａｔｉｎ、核糖基玉米素ＺＲ－ｚｅａｔｉｎｒｉｂｏｓｉｄｅ、二氢玉米素ＤＨＺ－ｄｉｈｙｄｒｏｚｅａｔｉｎ、核糖二氢玉米素ＤＨＺＲ－ｄｉｈｙｄｒｏｚｅａｔｉｎｒｉｂｏｓｉｄｅ、异戊烯基核糖基腺嘌呤ＩＰ－ｉｓｏｐｅｎｔｅｎｙｌａｄｅｎｉｎｅ和核糖基异戊烯基腺苷ＩＰＲ－ｉｓｏｐｅｎｔｅｎｙｌａｄｅｎｉｎｅｒｉｂｏｓｉｄｅ）含量进行定量分析．从用荧光显微技术分析ＤＮＡ含量变化结果看出，附加有激动素０．１×１０－６·Ｌ－１的悬浮液中，胡萝卜细胞的分裂周期为８．５ｈ，比不加外源激素的处理短１ｈ．在无外源激素存在的情况下，细胞分裂期中内源细胞分裂素类物质除ＺＲ之外，其余的Ｚ、ＤＨＺ、ＤＨＺＲ、ＩＰ和ＩＰＲ的含量在分裂的第６．５ｈ均有一个峰值出现，ＺＲ在第４．５和８．５ｈ有峰值出现．外加细胞分裂素的处理中．Ｚ、ｉＰ和ｉＰＲ整个周期无明显含量变化，ＤＨＺ和ＤＨＺＲ在第６．５ｈ出现峰值．ＺＲ在第８．５ｈ时也出现峰值．说明附加细胞激动素可协调Ｚ．ｉＰ和ｉＰＲ的积累，加速利用?

Investigations of Cytokinetin on the Cell Cycle in Carrot (Daucus carota L.) Cell Suspension

Using synchronization culture of cell cycle, high performance liquid chromatography(HPLC) and immunoenzymatic(ELISA) techniques, the DNA contents and endogenous cytokinetin levels:Zeann(Z), Zeatin Riboside(ZR), Dihydrozeatin (DHZ),Dihydrozeatin Riboside(DHZR), isopentenyladenine(iP), and isopentenyladenosine(iPR) were measured in carrot cells of cell cycle in plus kinetin (0.1×10-6·L-1) and without kinetin NL medium suspension. The result of DNA measured showed that the length of cell cycle in+kinetin suspension was 8. 5 h and in another-kinetin treatment was 9. 5 h. The contents of endogenous cytokinetin(Z, DHZ, DHZR,iP and iPR) appeared a high value in 6.5 h of cell cycle in without kinetin suspension, and the contents of ZR had two high values in 4. 5 and 8. 5 h similarly, the contents of DHZ and DHZR in+1 mg·L-1 kinetin suspension had a high value in 6.5 h of cell cycle,of ZR had only a high value in 8.5 h. But the contents of Z, iP and iPR had not appeared a hish value in cell cycle of carrot cells in+kinetin suspension. The results indicated that kinetin had apparently an effect on the accumulation and transposition of endogenous cytokinetin contents(Z, iP and iPR) in cell cycle.