| 兽用复方利巴韦林注射液组分含量色谱测定法 |
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| 引用本文： | 赵拉柱,武晋孝,宋艳红,王仲琴. 兽用复方利巴韦林注射液组分含量色谱测定法[J]. 山西农业科学, 2003, 31(2): 87-89 |
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| 作者姓名： | 赵拉柱 武晋孝 宋艳红 王仲琴 |
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| 作者单位： | 1. 山西省医药研究所,山西,太原,030006
2. 山西省兽药监察所,山西,太原,030027 |
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| 摘 要： | 采用高效液相色谱法测定兽用复方利巴韦林注射液各组分含量,以Extend-C18(十八烷基硅烷健合硅胶)为填充剂,水—乙腈—二乙胺(415∶85∶2.2,用磷酸调pH值为3.0)为流动相,流速1.0ml/min,检测波长225nm,进样量10μ1。该制剂利巴韦林在0.505～2.020μg、盐酸环丙沙星在0.510～2.040μg、乳酸甲氧苄胺嘧啶在0.250～1.000μg范围内峰面积与进样量呈线性关系,相关系数均为0.9999,平均回收率分别为99.9%,99.6%,99.5%。该方法分离效果好,准确可靠,适用于该产品质量控制。
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| 关 键 词： | 复方利巴韦林注射液 利巴韦林 盐酸环丙沙星 乳酸甲氧苄胺嘧啶 高效液相色谱法 含量测定 |
| 文章编号： | 1002-2481(2003)02-0087-03 |
| 修稿时间： | 2003-03-18 |
Determination of Ribavirin, Ciprofloxacini Hidrochloridum, Trimethoprim Lactate in Compound Ribavirin Inhection by HPLC |
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| Abstract: | To establish a method for determination of three constituents in compound ribavirin inhection:ribavirin,ciprofloxacini hidrochloridum and trimethoprim lactate by HPLC:Chromatograghic conditions were ZORBAX ExtendC 18 column(4.6mm×150mm,5μm);mobile phase water acetonitrilc triethylamine(415∶85∶2.2,adjust pH to 3.0 with H3PO4),;flow rate of 1.0ml/min.The UV detection wavelength was at 225 nm.Result:The linear response ranges were 0.505～2.020μg of ribavirin,0.510～2.040μg of ciprofloxacini hidrochloridum,0.250～1.000μg of trimethoprim lactate.The mean recoveries of ribavirin,ciprofloxacini hidrochloridum and trimethoprim lactate were:99.9%,99.6%,99.5%. Conclusion:The method shows good resolusion,satisfaetory accuracy.It is suitable for quality control of compound ribaverin inhection. |
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| Keywords: | HPLC Compound ribavirin inhection Ribavirin Ciprofloxacni hidrochloridum Trimethoprim lactate |
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