| 利用分子标记辅助选择改良温恢117白叶枯病抗性 |
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| 引用本文： | 罗天宽,张小玲,杨文清,卢华金,朱世杨,荆赞革,唐征,刘庆.利用分子标记辅助选择改良温恢117白叶枯病抗性[J].杂交水稻,2011,26(5). |
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| 作者姓名： | 罗天宽 张小玲 杨文清 卢华金 朱世杨 荆赞革 唐征 刘庆 |
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| 作者单位： | 温州科技职业学院,浙南作物育种重点实验室,浙江温州325006 |
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| 基金项目： | 温州市科技计划资助项目(N2006A001,N20090015) |
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| 摘 要： | 以恢复系温恢117为受体,以IRBB21为抗白叶枯病基因Xa21的供体,通过杂交、多次回交和自交,结合分子标记辅助选择技术,将Xa21基因导入温恢117中,结合抗性基因Xa21的分子检测和农艺性状表现,筛选出带有Xa21基因的15个恢复系株系.采用白叶枯病菌株的专化强毒菌系P6进行接种鉴定,各株系的白叶枯病抗性均强于受体亲本温恢117,其中株系M033抗性与IRBB21相当,而农艺性状与温恢117相近.
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| 关 键 词： | 水稻 白叶枯病抗性 分子标记辅助选择 |
Improvement of Bacterial Leaf Blight Resistance of Wenhui 117 by Molecular Marker-assisted Selection |
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| LUO Tian-kuan,ZHANG Xiao-ling,YANG Wen-qing,LU Hua-jin,ZHU Shi-yang,JING Zan-ge,TANG Zheng,LIU Qing.Improvement of Bacterial Leaf Blight Resistance of Wenhui 117 by Molecular Marker-assisted Selection[J].Hybrid Rice,2011,26(5). |
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| Authors: | LUO Tian-kuan ZHANG Xiao-ling YANG Wen-qing LU Hua-jin ZHU Shi-yang JING Zan-ge TANG Zheng LIU Qing |
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| Institution: | LUO Tian-kuan,ZHANG Xiao-ling,YANG Wen-qing,LU Hua-jin,ZHU Shi-yang,JING Zan-ge,TANG Zheng,LIU Qing(Wenzhou Vocational College of Science and Technology,Key Laboratory of Crop Breeding in South Zhejiang,Wenzhou,Zhejiang 325000,China) |
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| Abstract: | Using Wenhui 117,an elite restorer line in rice,as the receptor and IRBB21 as the donor of a gene to bacterial leaf blight(BLB) resistance,the resistant gene Xa21 was introduced into rice restorer lines through crossing and backcrossing with the molecular marker-assisted selection.15 restorer lines carrying the gene Xa21 were acquired based on the molecular marker test and agronomic performance.A subsequent inoculation identification of BLB resistance was conducted to these improved lines with the gene Xa21... |
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| Keywords: | rice bacterial leaf blight resistance molecular marker-assisted selection |
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