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| 文心兰花发育相关基因OAP3的克隆与表达分析 | |
| 引用本文： | 徐小雁,田敏,王彩霞,龙明华.文心兰花发育相关基因OAP3的克隆与表达分析[J].浙江林学院学报,2011,28(6):900-906. |
| 作者姓名： | 徐小雁 田敏 王彩霞 龙明华 |
| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,浙江富阳311400;广西大学农学院,广西南宁530003 2. 中国林业科学研究院亚热带林业研究所,浙江富阳,311400 3. 广西大学农学院,广西南宁,530003 |
| 基金项目： | 国家林业局引进国际先进农业科学技术计划("948"计划)项目，浙江省花卉产业创新团队项目 |
| 摘 要： | |
| 关 键 词： | 分子生物学 文心兰 花器官形成 MADS-box基因 OAP3基因 qRT-PCR |
Cloning and expression analysis of a flowering-related gene,OA P3 , in Oncidium |
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| XU Xiao-yan,TIAN Min,WANG Cai-xia,LONG Ming-hua.Cloning and expression analysis of a flowering-related gene,OA P3 , in Oncidium[J].Journal of Zhejiang Forestry College,2011,28(6):900-906. | |
| Authors: | XU Xiao-yan TIAN Min WANG Cai-xia LONG Ming-hua |
| Institution: | XU Xiao-yan1,2,TIAN Min1,WANG Cai-xia1,LONG Ming-hua2(1.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400,Zhejiang,China,2.Agricultural College,Guangxi University,Nanning 530003,Guangxi,China) |
| Abstract: | To investigate the expression pattern of MADS-box gene during the development of the orchids flower,the floral organ identity gene OAP 3 with a MADS-box motif was cloned from an orchid plant through the RT-PCR,Oncidium'Milliongolds'(GenBank accession No.GU644447).Then a sequence analysis and a phylogenetic analysis were conducted along with a quantitative real-time polymerase chain reaction(qRT-PCR).The full length cDNA of OAP 3 was 799 bp with an open reading frame(ORF) encoding 204 amino acids.The deduced... |
| Keywords: | molecular biology Oncidium'Milliongolds' floral initiation MADS-box genes OAP 3 gene qRT-PCR |
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