高大毛霉液体深层培养研究

利用豆腐生产工艺中的副产物黄泔水为溶剂,通过单因素实验和正交实验,在28℃、200 r/min摇瓶培养条件下,确定高大毛霉MHC-7最佳培养基为黄豆粉3%、可溶性淀粉0.4%、硫酸镁0.2%、磷酸二氢钾0.2%、蔗糖0.3%和黄泔水。培养基中碳氮比为1.5、最适接种量为6%、最适起始pH值为6.0,通过生物量测定,MHC-7液体培养在36 h达到最大值(1.363 g/100 mL)。

Study on Liquid Fermentation of Mucor mucedo (L.) Fres

Using yellow hogwash,the byproduct in the production of fermented tofu,as the solvent,via the single element and orthogonal experiment,the optimum medium of Mucor mucedo(L.) Fres is determined: shaking with 28 ℃,200 r/min,3% soybean powder,0.4% soluble starch,0.2% magnesium sulfate,0.2% potassium dihydrogen phosphate,0.3% sucrose and yellow hogwash.The adequate C∶N ratio in medium is 1.5.The initial optimal inoculum is 6% and pH is 6.0.Through biomass determination,the fermentation in liquid reached the maximum value in the 36 hour(1.363 g/100 mL).